Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis
| Autor: | Rankothge Ranjith Weerasiri, Shaowen Ji, Wei Wang, Ilsoon Lee, Yan Liu, Jue Lu |
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| Rok vydání: | 2013 |
| Předmět: |
Bioengineering
Cellulase Lignin Zea mays Applied Microbiology and Biotechnology Physical Phenomena Hydrolysis Biomass Food science Trichoderma reesei Trichoderma biology Chemistry beta-Glucosidase Aspergillus niger food and beverages biology.organism_classification Xylosidases Corn stover Biochemistry Fiber cell biology.protein Xylanase Fermentation Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 110:2123-2130 |
| ISSN: | 0006-3592 |
| DOI: | 10.1002/bit.24883 |
| Popis: | Cellulase, xylanase, and β-glucosidase production was studied on novel nano-shear pretreated corn stover by the mixed fungi culture. The high shear force from a modified Tayor-Couette nano-shear mixing reactor efficiently disintegrated corn stover, resulting in a homogeneous watery mash with particles in much reduced size. Scanning electron microscope study showed visible mini-pores on the fiber cell wall surface, which could improve the accessibility of the pretreated corn stover to microorganisms. Mixed fungal culture of Trichoderma reesei RUT-C30 and Aspergillus niger produced enzymes with higher cellulolytic and xylanolytic activities on corn stover pretreated with nano-shear mixing reactor, in comparison with other pretreatment methods, including acid and ammonia fiber explosion (AFEX) pretreatment. The hydrolytic potential of the whole fermentation broth from the mixed fungi was studied, and the possibility of applying the whole cell saccharification concept was also investigated to further reduce the cost of lignocellulose hydrolysis. Biotechnol. Bioeng. 2013; 110: 2123–2130. © 2013 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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