Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction
| Autor: | Sophie G. Martin, Daniela Gallo Castro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell type GTPase-activating protein GTP' Mitosis CDC42 GTPase macromolecular substances Article 03 medical and health sciences 0302 clinical medicine Cell polarity Schizosaccharomyces cdc42 GTP-Binding Protein Research Articles 030304 developmental biology 0303 health sciences biology Chemistry GTPase-Activating Proteins Cell Biology biology.organism_classification Sexual reproduction Cell biology 030104 developmental biology Cdc42 GTP-Binding Protein GTPase-Activating Proteins/genetics GTPase-Activating Proteins/metabolism Guanosine Triphosphate/genetics Guanosine Triphosphate/metabolism Mitosis/physiology Schizosaccharomyces/enzymology Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins/genetics Schizosaccharomyces pombe Proteins/metabolism cdc42 GTP-Binding Protein/genetics cdc42 GTP-Binding Protein/metabolism Schizosaccharomyces pombe Guanosine Triphosphate Schizosaccharomyces pombe Proteins biological phenomena cell phenomena and immunity 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Cell Biology The Journal of cell biology, vol. 217, no. 12, pp. 4215-4229 |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Local activity of the small GTPase Cdc42 is critical for cell polarization. Gallo Castro and Martin describe a new Cdc42 GTPase-activating protein (GAP) Rga3, which together with two other GAPs, constrains Cdc42-GTP zones during mitotic cycles but not during sexual reproduction. The formation of a local zone of Cdc42 GTPase activity, which governs cell polarization in many cell types, requires not only local activation but also switch-off mechanisms. In this study, we identify Rga3, a paralog of Rga4, as a novel Cdc42 GTPase-activating protein (GAP) in the fission yeast Schizosaccharomyces pombe. Contrary to Rga4, Rga3 localizes with Cdc42-GTP to sites of polarity. Rga3 is dispensable for cell polarization during mitotic growth, but it limits the lifetime of unstable Cdc42-GTP patches that underlie cell pairing during sexual reproduction, masking a partly compensatory patch-wandering motion. In consequence, cells lacking rga3 hyperpolarize and lose out in mating competition. Rga3 synergizes with the Cdc42 GAPs Rga4 and Rga6 to restrict Cdc42-GTP zone sizes during mitotic growth. Surprisingly, triple-mutant cells, which are almost fully round, retain pheromone-dependent dynamic polarization of Cdc42-GTP, extend a polarized projection, and mate. Thus, the requirement for Cdc42-GTP hydrolysis by GAPs is distinct during polarization by intrinsic or extrinsic cues. |
| Databáze: | OpenAIRE |
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