DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS
| Autor: | A. A. Babajanz, I. E. Efremova, S. B. Cheknev, E. N. Yushkovets, L. S. Piskovskaya |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
biology
Chemistry il-1β medicine.medical_treatment Immunology γ-globulin chemistry.chemical_element metal complexes Zinc RC581-607 Pha stimulation Copper Cytokine Biochemistry biology.protein medicine Immunology and Allergy Molecule Inducer production Immunologic diseases. Allergy Antibody Receptor induction |
| Zdroj: | Medicinskaâ Immunologiâ, Vol 12, Iss 6, Pp 497-502 (2014) |
| ISSN: | 2313-741X 1563-0625 |
| Popis: | Present study has shown that a total cytokine pool produced by human blood cells in presence of proteins from the γ-globulin fraction, or their complexes with copper and zinc, as well as with pure copper and zinc cations, contains up to 148.0±14.9 pg interleukin-1β (IL-1β) per mL. The γ-globulins modified by copper or zinc binding induce, resp., 2.0 and 1.4-fold less IL-1β than intact proteins (significant by p = 0.1). Meanwhile, the copper-γ-globulin proved to be 2.7-fold more active (р < 0.01) IL-1β inducer, as a zinc-protein complex. Pure zinc ions did not induce IL-1β production, whereas induction rates with pure copper ions are 1.6-fold higher (p < 0.05) than with γ-globulin, and 1.45-fold more efficient that PHA stimulation (p < 0.1). A potential participation of copper and zinc cations bound to antibodies from the microenvironment and, thus, transforming Fc regions of antibody molecules, is discussed with regard of regulation of IL-1β production by human blood cells induced by activation of their Fc receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|