Characterization of novel pollen-expressed transcripts reveals their potential roles in pollen heat stress response inArabidopsis thaliana
| Autor: | Karen Schlauch, Javier Forment, Tirza Doniger, Laetitia Poidevin, Jeffery F. Harper, Abhishek Rath, Alejandro Ferrando, Richard L. Tillett, Nicholas Rutley, Gad Miller, Gilad Luria |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Arabidopsis Plant Science Biology medicine.disease_cause 01 natural sciences Translatome Homology (biology) Transcriptome 03 medical and health sciences Intergenic region Pollen medicine Arabidopsis thaliana ORFS Gene 030304 developmental biology Genetics 0303 health sciences Phylogenetic tree RNA Cell Biology biology.organism_classification Heat Long non-coding RNA Open reading frame Original Article 010606 plant biology & botany Long noncoding RNA |
| Zdroj: | Plant Reproduction Digital.CSIC. Repositorio Institucional del CSIC instname |
| DOI: | 10.1101/2020.08.20.258764 |
| Popis: | Key message Arabidopsis pollen transcriptome analysis revealed new intergenic transcripts of unknown function, many of which are long non-coding RNAs, that may function in pollen-specific processes, including the heat stress response. Abstract The male gametophyte is the most heat sensitive of all plant tissues. In recent years, long noncoding RNAs (lncRNAs) have emerged as important components of cellular regulatory networks involved in most biological processes, including response to stress. While examining RNAseq datasets of developing and germinating Arabidopsis thaliana pollen exposed to heat stress (HS), we identified 66 novel and 246 recently annotated intergenic expressed loci (XLOCs) of unknown function, with the majority encoding lncRNAs. Comparison with HS in cauline leaves and other RNAseq experiments indicated that 74% of the 312 XLOCs are pollen-specific, and at least 42% are HS-responsive. Phylogenetic analysis revealed that 96% of the genes evolved recently in Brassicaceae. We found that 50 genes are putative targets of microRNAs and that 30% of the XLOCs contain small open reading frames (ORFs) with homology to protein sequences. Finally, RNAseq of ribosome-protected RNA fragments together with predictions of periodic footprint of the ribosome P-sites indicated that 23 of these ORFs are likely to be translated. Our findings indicate that many of the 312 unknown genes might be functional and play a significant role in pollen biology, including the HS response. |
| Databáze: | OpenAIRE |
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