A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity
| Autor: | Yusen Zhou, Shibo Jiang, Lanying Du, Guangyu Zhao, Lin Li, CC Chan, Yuxian He, Bo-Jian Zheng |
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| Rok vydání: | 2010 |
| Předmět: |
Antigenicity
Protein subunit Immunology Biology medicine.disease_cause SARS Virus - immunology law.invention Viral Envelope Proteins - chemistry - immunology Immune system Receptors Virus - metabolism law Virology medicine skin and connective tissue diseases Coronavirus Vaccines Chinese hamster ovary cell Viral Vaccine fungi Membrane Glycoproteins - chemistry - immunology body regions Recombinant DNA biology.protein Molecular Medicine Antibody Severe Acute Respiratory Syndrome - immunology - prevention and control |
| Zdroj: | Viral Immunology |
| ISSN: | 1557-8976 0882-8245 |
| DOI: | 10.1089/vim.2009.0090 |
| Popis: | Development of vaccines is essential for the prevention of future recurrences of severe acute respiratory syndrome (SARS), caused by the SARS coronavirus (SARS-CoV). The spike (S) protein, especially receptor-binding domain (RBD) of SARS-CoV, plays important roles in the prevention of SARS infection, and is thus an important component in SARS vaccine development. In this study, we expressed a 219-mer (residues 318-536) RBD protein in Chinese hamster ovary (CHO)-K1 cells (RBD219-CHO), and tested its immune responses and protective immunity in a mouse model. The results showed that this recombinant protein was correctly folded, being able to maintain intact conformation and authentic antigenicity. It could induce strong humoral and cellular immune responses and high titers of neutralizing antibodies in the vaccinated mice. RBD219-CHO protein elicited potent protective immunity that protected all vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD219-CHO protein has great potential for the development of an effective and safe SARS subunit vaccine. © Copyright 2010, Mary Ann Liebert, Inc.. published_or_final_version |
| Databáze: | OpenAIRE |
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