ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier
Autor: | Sunkyu Choi, Jie Jia, Intaek Lee, Piliang Hao, Yijing Wang, Bopil Gim, Shuaiyang Jing, Chuanting Tan, Mengjing Bao, Pascal Ziltener, Lianhui Zhu, Francesca Bottanelli, Yi Qian, Xihua Yue |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Receptors
Peptide Physiology QH301-705.5 KDEL ACBD3 ArfGAPs Golgi Apparatus Cellular homeostasis Plant Science Biology General Biochemistry Genetics and Molecular Biology symbols.namesake KDEL receptor Structural Biology Cell surface receptor Golgi Protein Kinase A Secretion Biology (General) Receptor Protein kinase A Ecology Evolution Behavior and Systematics Secretory pathway Adaptor Proteins Signal Transducing Arf1-GTP Cell Membrane 500 Naturwissenschaften und Mathematik::570 Biowissenschaften Biologie::570 Biowissenschaften Biologie Cell Biology Golgi apparatus Cyclic AMP-Dependent Protein Kinases Cell biology symbols General Agricultural and Biological Sciences Research Article Developmental Biology Biotechnology |
Zdroj: | BMC Biology, Vol 19, Iss 1, Pp 1-24 (2021) BMC Biology |
Popis: | Background KDEL receptor helps establish cellular equilibrium in the early secretory pathway by recycling leaked ER-chaperones to the ER during secretion of newly synthesized proteins. Studies have also shown that KDEL receptor may function as a signaling protein that orchestrates membrane flux through the secretory pathway. We have recently shown that KDEL receptor is also a cell surface receptor, which undergoes highly complex itinerary between trans-Golgi network and the plasma membranes via clathrin-mediated transport carriers. Ironically, however, it is still largely unknown how KDEL receptor is distributed to the Golgi at steady state, since its initial discovery in late 1980s. Results We used a proximity-based in vivo tagging strategy to further dissect mechanisms of KDEL receptor trafficking. Our new results reveal that ACBD3 may be a key protein that regulates KDEL receptor trafficking via modulation of Arf1-dependent tubule formation. We demonstrate that ACBD3 directly interact with KDEL receptor and form a functionally distinct protein complex in ArfGAPs-independent manner. Depletion of ACBD3 results in re-localization of KDEL receptor to the ER by inducing accelerated retrograde trafficking of KDEL receptor. Importantly, this is caused by specifically altering KDEL receptor interaction with Protein Kinase A and Arf1/ArfGAP1, eventually leading to increased Arf1-GTP-dependent tubular carrier formation at the Golgi. Conclusions These results suggest that ACBD3 may function as a negative regulator of PKA activity on KDEL receptor, thereby restricting its retrograde trafficking in the absence of KDEL ligand binding. Since ACBD3 was originally identified as PAP7, a PBR/PKA-interacting protein at the Golgi/mitochondria, we propose that Golgi-localization of KDEL receptor is likely to be controlled by its interaction with ACBD3/PKA complex at steady state, providing a novel insight for establishment of cellular homeostasis in the early secretory pathway. |
Databáze: | OpenAIRE |
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