Non-random binding of N-acetoxy-N-2-acetylamino-fluorene to chromatin subunits as visualized by immunoelectron microscopy
| Autor: | G. de Murcia, Leng M, Robert P. P. Fuchs, Michel Daune, M. C. Lang, A. Mazen |
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| Přispěvatelé: | Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 1982 |
| Předmět: |
Erythrocytes
Macromolecular Substances Immunoelectron microscopy Population MESH: Microscopy Electron Acetoxyacetylaminofluorene Toxicology MESH: Acetoxyacetylaminofluorene MESH: Chromatin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine polycyclic compounds Animals MESH: Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Binding site education 030304 developmental biology 0303 health sciences education.field_of_study Binding Sites [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Chemistry MESH: 2-Acetylaminofluorene MESH: Erythrocytes MESH: Chickens General Medicine MESH: Macromolecular Substances 2-Acetylaminofluorene DNA extraction Molecular biology Chromatin 3. Good health Microscopy Electron MESH: Binding Sites 030220 oncology & carcinogenesis Immunologic Techniques MESH: Immunologic Techniques Chickens Linker DNA |
| Zdroj: | Chem Biol Interact Chem Biol Interact, 1982, 41 (1), pp.83-93. ⟨10.1016/0009-2797(82)90019-9⟩ |
| Popis: | The influence of chromatin structure on the accessibility of DNA to the model ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene (N-Aco-AAF) was investigated by means of an immunoelectron microscopic technique developed recently. An homogeneous population of core particles or trinucleosomes from chicken erythrocytes, was submitted to electrophilic attack by N-Aco-AAF. After DNA isolation, N-2-acetylaminofluorene (AAF) binding sites were mapped upon the DNA fragments using specific antibodies as a probe. Our results indicate a non-random binding of AAF along the DNA. Our data support the results of previous studies showing a preferential binding on the linker region. |
| Databáze: | OpenAIRE |
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