Acute hypertriglyceridaemia in humans increases the triglyceride content and decreases the anti-inflammatory capacity of high density lipoproteins
| Autor: | Roland Stocker, Shirley Nakhla, Gilles Lambert, Rajesh Puranik, David S. Celermajer, Xiao S. Wang, Kerry Ann Rye, Stephen J. Nicholls, Sanjay Patel, Philip J. Barter, Pia Lundman |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Fat Emulsions Intravenous medicine.medical_specialty medicine.medical_treatment Vascular Cell Adhesion Molecule-1 Inflammation Random Allocation chemistry.chemical_compound Internal medicine medicine Humans Infusions Intravenous Saline Cells Cultured Triglycerides Hypertriglyceridemia Apolipoprotein A-I Triglyceride Tumor Necrosis Factor-alpha business.industry Vascular disease Endothelial Cells Intercellular Adhesion Molecule-1 Postprandial Period medicine.disease Up-Regulation Endocrinology medicine.anatomical_structure chemistry Acute Disease Tumor necrosis factor alpha Inflammation Mediators medicine.symptom Lipoproteins HDL Cardiology and Cardiovascular Medicine business Lipoprotein Artery |
| Zdroj: | Atherosclerosis. 204:424-428 |
| ISSN: | 0021-9150 |
| Popis: | Post-prandial hypertriglyceridaemia is a risk factor for cardiovascular disease, although the underlying mechanisms remain unclear. High density lipoproteins (HDL) have been shown to be atheroprotective, in part through attenuation of vascular inflammation. In this study, the influence of acute hypertriglyceridaemia on the composition and anti-inflammatory properties of HDL was investigated.Eight fasting healthy male subjects (34+/-2 years) received 20% Intralipid (15 mg/kg/h) or saline, on separate occasions in random order. At baseline and 60 min post-infusion, the total HDL fraction was isolated and its chemical composition determined. HDL were added to TNF-alpha stimulated human coronary artery endothelial cells and VCAM-1 and ICAM-1 expression was determined by flow cytometry.Serum triglyceride (97.4+/-8.5mg/dL baseline, 283.2+/-35.4 mg/dL post-infusion, p0.001) and HDL triglyceride content (3.8+/-0.5% HDL mass baseline, 5.3+/-0.9% HDL mass post-infusion, p0.05) increased significantly after Intralipid infusion. HDL post-Intralipid were significantly less anti-inflammatory compared with control (e.g. at 8 microM apoA-I, %VCAM-1 expression 54+/-5 post-saline, 73+/-4 post-Intralipid, p=0.01; %ICAM-1 expression 94+/-1 post-saline, 99.4+/-0.6 post-Intralipid, p0.01). There was also a significant correlation between HDL triglyceride content and VCAM-1 expression (R=0.70, p=0.005); as well as between plasma triglyceride levels and both VCAM-1 (R=0.71, p0.005) and ICAM-1 expression (R=0.80, p0.005).Acute hypertriglyceridaemia, simulating the post-prandial state, results in triglyceride-rich HDL with impaired anti-inflammatory capacity. |
| Databáze: | OpenAIRE |
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