Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells
| Autor: | Huang Handong, Zheng Xingbin, Jie Xu, Xie Ming, Yang Xuefeng, Wang Maijian |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Cancer Research Apoptosis Endocrine Disruptors Biology Biochemistry Phosphatidylinositol 3-Kinases 03 medical and health sciences 0302 clinical medicine Phenols Transforming Growth Factor beta Cell Line Tumor Proto-Oncogene Proteins Genetics Humans Phosphorylation Molecular Biology PI3K/AKT/mTOR pathway Cell Proliferation Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Estradiol Oncogene Cell growth Kinase Intracellular Signaling Peptides and Proteins G1 Phase Cell Cycle Checkpoints Molecular biology Up-Regulation 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research Molecular Medicine Colorectal Neoplasms Proto-Oncogene Proteins c-fos A431 cells Signal Transduction Transforming growth factor |
| Zdroj: | Molecular Medicine Reports. 16:2211-2216 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2017.6817 |
| Popis: | Nonylphenol (NP) is a well-known endocrine-disrupting chemical (EDC), which can enhance the progression of cancer by functioning as an estrogen‑like factor. In the present study, the effects of different concentrations of NP on COLO205 colorectal cancer (CRC) cells were examined. The results of flow cytometric analysis revealed that NP significantly decreased the proportion of cells in the G0/G1 phase in a dose‑dependent manner, which was accompanied by a marginal increase in the proportions of cells in S and G2/M phases. NP did not induce apoptosis, whereas estradiol (E2) did induce apoptosis. To elucidate the mechanisms underlying the action of NP on COLO205 cells, the transcriptional levels of extracellular signal‑regulated kinase (ERK)1, ERK2 and phosphoinositide 3‑kinase (PI3K) were assessed using reverse transcription‑quantitative polymerase chain reaction analysis. The expressions levels of ERK1, ERK2 and PI3K were increased by treatment with NP in a dose‑dependent manner. On examining protein levels, the expression of PI3K p38 was increased by NP and E2, and the expression of ERK1/2 was increased by NP. The phosphorylation of the ERK protein was significantly increased by treatment with NP at a high concentration (10‑4 M; P |
| Databáze: | OpenAIRE |
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