Production of intracellular and extracellular interleukin-1 alpha and interleukin-1 beta by peripheral blood monocytes from patients with connective tissue diseases
| Autor: | R Yokohari, T Nakano, M Okawa-Takatsuji, S Aotsuka, M Kinoshita, Kawakami M, Makoto Goto, K Nakamura |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Male medicine.medical_specialty Immunology Connective tissue Alpha (ethology) Enzyme-Linked Immunosorbent Assay Monocytes General Biochemistry Genetics and Molecular Biology Mixed connective tissue disease Rheumatology Internal medicine Extracellular medicine Humans Lupus Erythematosus Systemic Immunology and Allergy Connective Tissue Diseases Beta (finance) Scleroderma Systemic business.industry Interleukin Middle Aged medicine.disease Connective tissue disease Sjogren's Syndrome Endocrinology medicine.anatomical_structure Ribonucleoproteins Antibodies Antinuclear Female business Intracellular Interleukin-1 Research Article |
| Zdroj: | Annals of the Rheumatic Diseases. 50:27-31 |
| ISSN: | 0003-4967 |
| DOI: | 10.1136/ard.50.1.27 |
| Popis: | An enzyme linked immunosorbent assay (ELISA) was used to estimate the production of intracellular and extracellular interleukin-1 (IL-1) alpha and beta by peripheral blood monocytes from 26 patients with various connective tissue diseases (CTD), including 19 with systemic lupus erythematosus, four with progressive systemic sclerosis, two with mixed connective tissue disease, and one with Sjögren's syndrome. Monocytes obtained from patients with CTD with serum antibodies to nuclear ribonucleoprotein (nRNP) released significantly higher concentrations of extracellular IL-1 alpha and IL-1 beta, whereas intracellular IL-1 alpha and IL-1 beta production was similar to that by monocytes from patients with CTD without antibodies to nRNP. Furthermore, the concentrations of extracellular IL-1 alpha correlated significantly with those of extracellular IL-1 beta. There was no significant correlation between the concentrations of extracellular and intracellular IL-1 alpha, and those of extracellular and intracellular IL-1 beta, indicating that synthesis and secretion of IL-1 by human monocytes may be two distinct biological events. It seems that enhanced extracellular release of both IL-1 alpha and IL-1 beta contributes to the excessive anti-nRNP production in CTD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|