Efficacy of Aeschynomene indica L. leaves for wound healing and isolation of active constituent
| Autor: | Mingzhang Ao, Zhi-Jian Cao, Longjiang Yu, Jing-Jing Chen, Zhi-Yong Lei |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Pathology medicine.medical_specialty Angiogenesis Dalbergia H&E stain 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Drug Discovery medicine Animals Rats Wistar Fibroblast Skin 030304 developmental biology Pharmacology Wound Healing 0303 health sciences integumentary system Eosin Plant Extracts CD68 business.industry Macrophages Staining Plant Leaves medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Wound healing business Phytotherapy |
| Zdroj: | Journal of Ethnopharmacology. 228:156-163 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2018.08.008 |
| Popis: | Ethnopharmacological relevance In traditional Chinese medicine, the aerial parts of Aeschynomene indica L. (AIL) have been used for wound healing, and to treat urinary tract infection, hepatitis, enteritis, dysentery, nyctalopia, conjunctivitis, urticaria, and furuncle. However, no scientific investigation has been conducted on its wound healing potential. Aim of the study To investigate the effects of AIL extract on wound healing, isolate the active constituent and reveal the possible mechanism of enhancing wound healing. Materials and methods The circular excision wound healing model was used to evaluate in vivo wound-healing activity. Hematoxylin and eosin staining was applied to assess inflammatory cells infiltration, angiogenesis, fibroblast proliferation, collagen synthesis, collagen remodeling, and skin appendages generation. Sirius red-picric acid staining was employed for quantitative analysis of the ratio of collagen I/III. Immunohistochemical staining for CD68, CCR7 (CD197), CD163, TGF-β1 and α-SMA was performed to determine macrophages phenotypes transition (M1-to-M2) and prove the scar-improving effect of AIL on wound healing. Results We successfully isolated the active constituent (Sub-Fr0.2) for wound healing from AIL extract, circular excision wound healing experiment and hematoxylin & eosin staining showed Sub-Fr0.2 has a significant promoting effect on wound healing. Results of sirius red-picric acid staining demonstrated a reduced ratio of collagen I/III in the Sub-Fr0.2 group as compared with the vehicle group. Immunohistochemical staining for CD68, CCR7 (CD197), and CD163 in the Sub-Fr0.2 group exhibited an elevated speed of macrophages transiting from M1 phenotype to M2 phenotype, when compared with the vehicle group. Besides, the expression of TGF-β1 and α-SMA were inhibited on wounds treated with the ointment containing Sub-Fr0.2. Conclusion Leaves of AIL and its active constituent (Sub-Fr0.2) effectively promoted wound healing and reduced scar formation, this efficacy might be exerted by accelerating macrophages phenotypes transition and inhibiting TGF-β1 and α-SMA expression. |
| Databáze: | OpenAIRE |
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