Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset)
| Autor: | Chisei Ra, Yoshie Kametani, Manabu Ozawa, Kenzaburo Tani, Toshio Itoh, Kiyoshi Ando, Azumi Kono, Yuko Yamada, Hideo Yagita, Sonoko Habu, Erika Sasaki, Mino Yoshioka, Shin Shimada, Ryuji Suzuki, Masanobu Satake, Satoshi Nunomura, Hiroshi Suemizu |
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| Rok vydání: | 2012 |
| Předmět: |
Immunology
CD34 Antigens CD34 Bone Marrow Cells Stem cell factor Tryptase Cell Separation Mice SCID Mice Mice Inbred NOD medicine Animals Humans Immunology and Allergy Mast Cells Progenitor cell Cells Cultured Cell Proliferation Mice Knockout biology Receptors IgE Stem Cells Callithrix Cell Differentiation General Medicine Flow Cytometry biology.organism_classification Mast cell Cell biology Proto-Oncogene Proteins c-kit Haematopoiesis medicine.anatomical_structure Models Animal biology.protein Bone marrow Biomarkers |
| Zdroj: | International Immunology. 24:593-603 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/dxs070 |
| Popis: | Mast cells (MCs) are developed from hematopoietic progenitor cells and play an important role in inflammation. Study of the kinetics of development and accumulation of primate MC in vivo is crucial for the control of human inflammatory diseases, as evolution of the immune system is quite rapid and inflammation including MC response is considered to be different between mouse and human. In the present study, we examined the development of MC from hematopoietic progenitors of Callithrix jacchus (common marmoset), an experimental animal of nonhuman primates. Bone marrow cells were fractionated for the expression of CD34 and CD117 by cell sorting. MCs were developed in vitro or by transplanting the cells to NOD/SCID/IL-2γc knockout (NOG) mice. In vitro culture of CD34(+)CD117(+) (double positive, DP) cells with stem cell factor could generate high-affinity Fc epsilon receptor (FcεR)-expressing CD117(+) cells with typical granules. The developed MC released β-hexosaminidase and produced leukotriene C(4) after the stimulation of FcεRI. Transplantation of DP cells gave rise to a marked expansion of CD34(-)CD45(+)CD117(+)FcεR(+) cells in NOG mice. They expressed transcripts encoding chymase 1 and tryptase β. Differentiation of CD34(-)CD117(+) cells to MCs was relatively limited compared with the DP cells, similarly to human MCs. These results suggest that this marmoset system provides a good model for human MC development. |
| Databáze: | OpenAIRE |
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