Histopathological and immunohistochemical studies on arteritis induced by fenoldopam, a vasodilator, in rats
| Autor: | Takanori Hanada, Katsuhiko Ishida, Kunio Doi, Takao Shishido, Hisashi Ikegami, Hiroyuki Nakayama |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Pathology Time Factors Necrosis CD3 Complex Fenoldopam Vasodilator Agents Hemorrhage Inflammation Toxicology Pathology and Forensic Medicine Immunoenzyme Techniques Fibrosis Internal medicine Adventitia medicine Animals Arteritis Fluorescent Antibody Technique Indirect Infusions Intravenous Epidermal Growth Factor business.industry Vascular disease Cell Biology General Medicine Antigens CD20 medicine.disease Rats Inbred F344 Rats medicine.anatomical_structure Endocrinology Immunoglobulin M Immunoglobulin G Intercellular Signaling Peptides and Proteins medicine.symptom Tunica Media Splanchnic business Biomarkers Heparin-binding EGF-like Growth Factor medicine.drug |
| Zdroj: | Experimental and Toxicologic Pathology. 53:25-30 |
| ISSN: | 0940-2993 |
| DOI: | 10.1078/0940-2993-00158 |
| Popis: | Summary Fenoldopam, a dopaminergic (DA1) agonist, has been reported to induce medial necrosis and adventitial inflammatory response in the splanchnic arteries in rats. This study was carried out to clarify the detailed time course of the inflammatory responses, using antibodies for the inflammatory cell markers, CD3 (T cell), CD20 (B cell) and ED-1 (macrophage), and inflammatory serum factors, IgG, IgM and C3. Rats were administered fenoldopam for 24 hours by intravenous infusion. Histopathologically, medial necrosis with hemorrhage was observed at the end of infusion, but it almost disappeared on day 7 post-infusion. Adventitial inflammatory responses with ED-1-, CD3- and CD20-positive cells were very slight at the end of infusion, became prominent with marked fibrosis on days 3 and 5, decreased on day 7, and subsided on day 14. The serum factors were first present in the area of medial necrosis, then shifted to the subendothelial space or cytoplasm of smooth muscle cells, and disappeared on day 14 post-infusion. Gaps in the external elastic lamina were observed on days 3 and 5 post-infusion, and IgG and IgM were present outside the gaps in the adventitia. These results provided us with more detailed information on the inflammatory responses following medial damage induced by vasodilators. |
| Databáze: | OpenAIRE |
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