E6/E7 Variants of Human Papillomavirus 16 Associated with Cervical Carcinoma in Women in Southern Mexico
| Autor: | Marco Antonio Leyva-Vázquez, Berenice Illades-Aguiar, Oscar Del Moral-Hernández, Marco Antonio Jiménez-López, Julio Ortiz-Ortiz, Jorge Adán Navor-Hernández, Ramón Antaño-Arias, Jorge Organista-Nava, Luz del Carmen Alarcón-Romero |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Silent mutation medicine.medical_specialty cervical cancer Gastroenterology Article 03 medical and health sciences 0302 clinical medicine Internal medicine Cervical carcinoma medicine Odd ratio Immunology and Allergy Human papillomavirus Molecular Biology E7 E6 squamous intraepithelial lesion Cervical cancer E7 non-synonymous mutation General Immunology and Microbiology business.industry medicine.disease Confidence interval Squamous intraepithelial lesion human papillomavirus 16 030104 developmental biology Infectious Diseases Increased risk 030220 oncology & carcinogenesis Medicine business |
| Zdroj: | Pathogens Volume 10 Issue 6 Pathogens, Vol 10, Iss 773, p 773 (2021) |
| ISSN: | 2076-0817 |
| DOI: | 10.3390/pathogens10060773 |
| Popis: | Persistent infection with the human papillomavirus 16 (HPV 16) is the cause of half of all cervical carcinomas (CC) cases. Moreover, mutations in the oncoproteins E6 and E7 are associated with CC development. In this study, E6/E7 variants circulating in southern Mexico and their association with CC and its precursor lesions were evaluated. In total, 190 DNA samples were obtained from scrapes and cervical biopsies of women with HPV 16 out of which 61 are from patients with CC, 6 from patients with high-grade squamous intraepithelial lesions (HSIL), 68 from patients with low-grade squamous intraepithelial lesions (LSIL), and 55 from patients without intraepithelial lesions. For all E7 variants found, the E7-C732/C789/G795 variant (with three silent mutations) was associated with the highest risk of CC (odd ratio (OR) = 3.79, 95% confidence interval (CI) = 1.46–9.85). The analysis of E6/E7 bicistron conferred to AA-a*E7-C732/C789/G795 variants revealed the greatest increased risk of CC (OR = 110, 95% CI = 6.04–2001.3), followed by AA-c*E7-C732/C789/G795 and A176/G350*E7-p. These results highlight the importance of analyzing the combinations of E6/E7 variants in HPV 16 infection and suggest that AA-a*E7-C732/C789/G795, AA-c*E7-C732/C789/G795, and A176/G350*E7-p can be useful markers for predicting CC development. |
| Databáze: | OpenAIRE |
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