Induction of high-affinity paf receptor expression during T cell activation
| Autor: | Yolande Richard, Jacques Benveniste, Yolène Thomas, Momar Cheik Nguer, Olivier Pellegrini, Cynthia Calabresse |
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| Rok vydání: | 1992 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte medicine.medical_specialty Time Factors CD3 Complex CD3 T cell Receptor expression T-Lymphocytes Immunology Cell CD2 Antigens Receptors Antigen T-Cell Receptors Cell Surface Platelet Membrane Glycoproteins Lymphocyte Activation Receptors G-Protein-Coupled chemistry.chemical_compound Radioligand Assay Cell surface receptor Internal medicine medicine Immunology and Allergy Humans Platelet Activating Factor Receptors Immunologic Receptor Cells Cultured biology Platelet-activating factor Dose-Response Relationship Drug T lymphocyte respiratory system Molecular biology Up-Regulation medicine.anatomical_structure Endocrinology chemistry Gene Expression Regulation biology.protein Interleukin-2 lipids (amino acids peptides and proteins) |
| Zdroj: | European journal of immunology. 22(6) |
| ISSN: | 0014-2980 |
| Popis: | Activated human T cells via the CD2 or the CD3 pathways exhibited a higher capacity than resting T lymphocytes to incorporate and metabolize [3H]pafacether (paf) at 37 degrees C. Resting T lymphocytes lacked specific binding capacity for paf, yet high-affinity paf receptors (paf-R) were induced on CD3- or CD2-dependent activation. This up-regulation in the number of paf-R became apparent by day 1 of culture, reached a maximum of about 25,000 sites cell by days 4 to 6 and subsequently declined. Interestingly, human recombinant interleukin-2 in a dose-dependent manner prevented the decrease of high-affinity paf-R expression on T cells. By contrast, the receptor affinity was constant throughout the culture period. Thus, paf-R at different stages of T cell activation were indistinguishable with respect to receptor-ligand interaction, and differed only in their number. Together, these data demonstrate that after activation human T cells develop membrane high-affinity paf-binding sites. They also suggest for the first time that expression of the paf-R are coupled to T cell activation and/or differentiation. |
| Databáze: | OpenAIRE |
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