The influence of inhibiting no formation on metabolic recovery of ischemic rat heart by apelin-12
| Autor: | Irina Studneva, A. A. Az’muko, Maria Sidorova, Oleg I. Pisarenko, Shul'zhenko Vs, Pelogeĭkina IuA, Bespalova ZhD, Pal'keeva Me |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Cardiac function curve medicine.medical_specialty Myocardial Ischemia Energy metabolism Ischemia Myocardial Reperfusion Peptide In Vitro Techniques Nitric Oxide General Biochemistry Genetics and Molecular Biology Adenosine Triphosphate Adenine nucleotide Internal medicine medicine Animals Enzyme Inhibitors Rats Wistar Energy charge chemistry.chemical_classification Cardioprotection General Medicine medicine.disease Rats Apelin NG-Nitroarginine Methyl Ester Endocrinology chemistry Lactates Cardiology Intercellular Signaling Peptides and Proteins Isotonic Solutions Energy Metabolism |
| Zdroj: | Biomeditsinskaya Khimiya. 58:702-711 |
| ISSN: | 2310-6905 2310-6972 |
| DOI: | 10.18097/pbmc20125806702 |
| Popis: | Apelin 12 (A-12) was synthesized by the automatic solid phase method with use of Fmoc 1H-NMR spectroscopy and mass spectrometry. Effects of apelin-12 (a peptide comprised of 12 aminoacids, A-12) on recovery of energy metabolism and cardiac function were studied in isolated working rat hearts perfused with Krebs buffer (KB) containing 11 mM glucose that were subjected to global ischemia and reperfusion. A short-term infusion of microM 140 A-12 in KB prior to ischemia enhanced myocardial ATP, the total adenine nucleotide pool (SigmaAN = ATP + ADP + AMP) and the energy charge of cardiomyocites ((ATP + 0.5ADP)/SigmaAN) at the end of reperfusion compared with control (KB infusion) and reduced lactate content and lactate/pyruvate ratio in reperfused myocardium to the initial values. This effect was accompanied by improved recovery of coronary flow and cardiac function. Coadministration of 140 microM A-12 and 100 microM L-NAME (the nonspecific NOS inhibitor) profoundly attenuated the peptide influence on metabolic and functional recovery of reperfused hearts. The results indicate involvement of NO, formed under the peptide action, in mechanisms of cardioprotection that are tightly associated with recovery of energy metabolism in postischemic heart. |
| Databáze: | OpenAIRE |
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