Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays

Autor:	Robert Hartlage, Brock A. Peters, Igor Nazarenko, Jonathan Baccash, Calvin Kong, Vitali Karpinchyk, Andres Fernandez, Abraham M. Rosenbaum, Ryan J. Cedeno, Paolo Carnevali, Celeste E. McBride, Norman L. Burns, Shaunak Roy, Karen W. Shannon, George M. Church, Snezana Drmanac, Daniel F. Chernikoff, Radoje Drmanac, Geoffrey B. Nilsen, Claudia Richter, Coleen R. Hacker, Jay Shafto, William C. Banyai, Kaliprasad Pothuraju, Helena Perazich, Bruce L. Martin, Dennis G. Ballinger, Benjamin Curson, Linsu Chen, Brian Hauser, Steve Huang, Alexander Wait Zaranek, Anushka Brownley, Dylan Vu, Matt Morenzoni, Andrew B. Sparks, Matthew J. Callow, Alex Cheung, Clifford Reid, Adam P. Borcherding, George Yeung, Xiaodi Wu, Catherine Le, Tom Landers, Aaron L. Halpern, Bahram G. Kermani, Kimberly Perry, Arnold R. Oliphant, Mark Koenig, Charit L. Pethiyagoda, Michel Sun, Joseph V. Thakuria, Conrad G. Sheppy, Anne Tran, Robert E. Morey, Fredrik A. Dahl, Krishna Pant, Karl Mutch, Bryan Staker, Joe Peterson, Jessica Ebert, Yuan Jiang, Jia Liu, Razvan Chirita, Uladzislau Sharanhovich
Rok vydání:	2010
Předmět:	Male Genotype Sequence analysis Biology Polymorphism Single Nucleotide Genome DNA sequencing chemistry.chemical_compound Sequencing by hybridization Human Genome Project Humans Nanotechnology Genomic library Genetics Genomic Library Multidisciplinary Base Sequence Genome Human Computational Biology DNA Sequence Analysis DNA Nucleic acid amplification technique Microarray Analysis Nanostructures Haplotypes chemistry Costs and Cost Analysis Human genome Databases Nucleic Acid Nucleic Acid Amplification Techniques Software
Zdroj:	Science. 327:78-81
ISSN:	1095-9203 0036-8075
Popis:	Toward $1000 Genomes The ability to generate human genome sequence data that is complete, accurate, and inexpensive is a necessary prerequisite to perform genome-wide disease association studies. Drmanac et al. (p. 78 , published online 5 November) present a technique advancing toward this goal. The method uses Type IIS endonucleases to incorporate short oligonucleotides within a set of randomly sheared circularized DNA. DNA polymerase then generates concatenated copies of the circular oligonucleotides leading to formation of compact but very long oligonucleotides which are then sequenced by ligation. The relatively low cost of this technology, which shows a low error rate, advances sequencing closer to the goal of the $1000 genome.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfc5b869194526e702f24446172ea13f https://doi.org/10.1126/science.1181498 Zobrazit plný text záznamu Plný text ve formátu PDF