The bovine seminal plasma protein PDC-109 extracts phosphorylcholine-containing lipids from the outer membrane leaflet
| Autor: | Jürgen Schiller, Puttaswamy Manjunath, Karin Müller, Anke Kurz, Karl-Rudolf Erlemann, Peter Müller, Edda Töpfer-Petersen, Astrid Tannert, Andreas Herrmann |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Phosphorylcholine Biophysics Phospholipid macromolecular substances Biology Seminal Vesicle Secretory Proteins Membrane Lipids chemistry.chemical_compound Phosphatidylcholine Animals Humans Cells Cultured chemistry.chemical_classification Cell Membrane Fatty acid hemic and immune systems Biological membrane General Medicine Spermatozoa Sperm chemistry Biochemistry Liposomes Cattle lipids (amino acids peptides and proteins) Bacterial outer membrane Sphingomyelin Protein Binding |
| Zdroj: | European Biophysics Journal. 36:461-475 |
| ISSN: | 1432-1017 0175-7571 |
| DOI: | 10.1007/s00249-006-0105-3 |
| Popis: | The bovine seminal plasma protein PDC-109 modulates the maturation of bull sperm cells by removing lipids, mainly phosphatidylcholine and cholesterol, from their cellular membrane. Here, we have characterized the process of extraction of endogenous phospholipids and of their respective analogues. By measuring the PDC-109-mediated release of fluorescent phospholipid analogues from lipid vesicles and from biological membranes (human erythrocytes, bovine epididymal sperm cells), we showed that PDC-109 extracts phospholipids with a phosphorylcholine headgroup mainly from the outer leaflet of these membranes. The ability of PDC-109 to extract endogenous phospholipids from epididymal sperm cells was followed by mass spectrometry, which allowed us to characterize the fatty acid pattern of the released lipids. From these cells, PDC-109 extracted phosphatidylcholine and sphingomyelin that contained an enrichment of mono- and di-unsaturated fatty acids as well as short-chain and lyso-phosphatidylcholine species. Based on the results, a model explaining the phospholipid specificity of PDC-109-mediated lipid release is presented. |
| Databáze: | OpenAIRE |
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