Zebrafish B cell acute lymphoblastic leukemia: new findings in an old model
| Autor: | Ameera Hasan, Chiara Borga, Jessica Burroughs-Garcia, Clay A. Foster, Gilseung Park, J. Kimble Frazer |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Lymphocyte Transgene Danio acute lymphoblastic leukemia MYC lymphocyte 03 medical and health sciences 0302 clinical medicine biology.animal Gene expression medicine Zebrafish biology Cancer Vertebrate B-cell acute lymphoblastic leukemia zebrafish medicine.disease biology.organism_classification 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Research Perspective Cancer research ALL |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Acute lymphoblastic leukemia (ALL) is the most common pediatric, and ninth most common adult, cancer. ALL can develop in either B or T lymphocytes, but B-lineage ALL (B-ALL) exceeds T-ALL clinically. As for other cancers, animal models allow study of the molecular mechanisms driving ALL. Several zebrafish (Danio rerio) T-ALL models have been reported, but until recently, robust D. rerio B-ALL models were not described. Then, D. rerio B-ALL was discovered in two related zebrafish transgenic lines; both were already known to develop T-ALL. Here, we report new B-ALL findings in one of these models, fish expressing transgenic human MYC (hMYC). We describe B-ALL incidence in a large cohort of hMYC fish, and show B-ALL in two new lines where T-ALL does not interfere with B-ALL detection. We also demonstrate B-ALL responses to steroid and radiation treatments, which effect ALL remissions, but are usually followed by prompt relapses. Finally, we report gene expression in zebrafish B lymphocytes and B-ALL, in both bulk samples and single B- and T-ALL cells. Using these gene expression profiles, we compare differences between the two new D. rerio B-ALL models, which are both driven by transgenic mammalian MYC oncoproteins. Collectively, these new data expand the utility of this new vertebrate B-ALL model. |
| Databáze: | OpenAIRE |
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