A novel methanotroph in the genus Methylomonas that contains a distinct clade of soluble methane monooxygenase
| Autor: | Jong-Geol Kim, Hye-Young Yang, Seong Woon Roh, Man-Young Jung, Ngoc-Loi Nguyen, Sung-Keun Rhee, Soo-Je Park, Woon-Jong Yu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial Methanotroph Methane monooxygenase Nitrogen 030106 microbiology Fresh Water Methylomonas Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Phylogenetics Nitrogen Fixation RNA Ribosomal 16S Botany Republic of Korea Phylogeny biology Methanol dehydrogenase Base Sequence General Medicine Sequence Analysis DNA Ribosomal RNA 16S ribosomal RNA biology.organism_classification Carbon Bacterial Typing Techniques Alcohol Oxidoreductases 030104 developmental biology Genes Bacterial Anaerobic oxidation of methane Methylococcaceae biology.protein Oxygenases Water Microbiology Methane |
| Zdroj: | Journal of microbiology (Seoul, Korea). 55(10) |
| ISSN: | 1976-3794 |
| Popis: | Aerobic methane oxidation is a key process in the global carbon cycle that acts as a major sink of methane. In this study, we describe a novel methanotroph designated EMGL16-1 that was isolated from a freshwater lake using the floating filter culture technique. Based on a phylogenetic analysis of 16S rRNA gene sequences, the isolate was found to be closely related to the genus Methylomonas in the family Methylococcaceae of the class Gammaproteobacteria with 94.2–97.4% 16S rRNA gene similarity to Methylomonas type strains. Comparison of chemotaxonomic and physiological properties further suggested that strain EMGL16-1 was taxonomically distinct from other species in the genus Methylomonas. The isolate was versatile in utilizing nitrogen sources such as molecular nitrogen, nitrate, nitrite, urea, and ammonium. The genes coding for subunit of the particulate form methane monooxygenase (pmoA), soluble methane monooxygenase (mmoX), and methanol dehydrogenase (mxaF) were detected in strain EMGL16-1. Phylogenetic analysis of mmoX indicated that mmoX of strain EMGL16-1 is distinct from those of other strains in the genus Methylomonas. This isolate probably represents a novel species in the genus. Our study provides new insights into the diversity of species in the genus Methylomonas and their environmental adaptations. |
| Databáze: | OpenAIRE |
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