Simultaneous determination of eight flavonoids in Sedum sarmentosum Bunge from different areas by UHPLC with triple quadrupole MS/MS
| Autor: | Xue Wang, Zhitao Jiang, Jinhuo Pan, Chundi Liu, Jianchun Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Electrospray
Clinical Biochemistry Active components Tandem mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Sedum Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Drug Stability Limit of Detection Tandem Mass Spectrometry Drug Discovery Cluster Analysis Molecular Biology Chromatography High Pressure Liquid Pharmacology Flavonoids Chromatography Chemistry Plant Extracts 010401 analytical chemistry Reproducibility of Results General Medicine Repeatability 0104 chemical sciences Triple quadrupole mass spectrometer Sedum sarmentosum Chemical constituents Linear Models |
| Zdroj: | Biomedical chromatography : BMC. 33(9) |
| ISSN: | 1099-0801 |
| Popis: | Sedum sarmentosum Bunge (SSB) is a traditional Chinese herbal medicine containing multiple components that has been extensively used clinically to treat chronic viral hepatitis and some inflammatory diseases. Total flavonoids are major pharmacologically active components of SSB. To gain a deeper understanding of SSB resources, we analyzed eight chemical constituents in 33 batches of SSB from 11 regions in China. An accurate, precise and sensitive ultra-high-performance liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry method was developed for the determination of eight flavonoids in SSB. Under the optimized chromatographic conditions, good separation for the eight target components was obtained on an Agilent Zobax SB C18 (50 × 2.1 mm, 5 μm) column within 4 min. The established methods were validated with good linearity (r ≥ 0.9988), precision (RSD ≤ 2.68%), stability (1.43-3.28%) and repeatability (1.14-2.89%). Moreover, the average recoveries were 95.91-100.68%, and the RSDs were 1.50-3.80%. In addition, the analytical conditions of UPLC-ESI-MS/MS provided better sensitivity with a shorter analysis time when compared with the HPLC-DAD method. Hierarchical clustering analysis and principal component analysis were performed to estimate and classify these samples based on the contents of the eight chemical constituents. This study provided the theoretical basis and scientific evidence for the development and utilization of SSB resources. |
| Databáze: | OpenAIRE |
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