Characterization of the Pharmaceutical Effect of Drugs on Atherosclerotic Lesions in Vivo Using Integrated Fluorescence Imaging and Raman Spectral Measurements
| Autor: | Yi Cyun Yang, Shao Kang Huang, Wei-Tien Chang, Ian Liau |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Drug
Fluorescence-lifetime imaging microscopy media_common.quotation_subject Atorvastatin Hypercholesterolemia Pharmacology Spectrum Analysis Raman Analytical Chemistry Ezetimibe In vivo medicine Animals Pyrroles Zebrafish media_common Microscopy Confocal medicine.diagnostic_test Chemistry Anticholesteremic Agents Therapeutic effect Lipid metabolism Atherosclerosis Lipid Metabolism Lipids Heptanoic Acids Azetidines Blood Vessels lipids (amino acids peptides and proteins) Lipid profile medicine.drug |
| Zdroj: | Analytical Chemistry. 86:3863-3868 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac404051f |
| Popis: | Direct assessment of the vascular lesions of model animals in vivo is important for the development of new antiatherosclerotic drugs. Nevertheless, biochemical analysis of the lipid profile in blood in vitro remains the most common way to evaluate the therapeutic effect of drugs targeting atherosclerosis because of an inherent difficulty to access the vascular wall. Using hypercholesterolemic zebrafish, we present an orchestrated application of Raman spectral measurements and confocal fluorescence imaging to interrogate the pharmacological response of atherosclerotic lesions in situ and in vivo. For demonstration, we investigated two commonly prescribed antihyperlipidemic drugs, ezetimibe and atorvastatin. The treatment of ezetimibe or atorvastatin alone decreased effectively the deposition of lipids in the vascular wall, and a combined dose showed a synergistic effect. Atorvastatin exerted a profound antioxidative effect on vascular fatty lesions. Analysis of individual lesions shows further that these lesions exhibited a heterogeneous response to the treatment of atorvastatin; a significant fraction of, but not all, the lesions became nonoxidized after the intervention. Beyond its efficacies in suppressing both the accumulation and oxidation of vascular lipids, atorvastatin expedited the clearance of vascular lipids. The possession of pleotropic (multiple) therapeutic effects on vascular fatty lesions of hypercholesterolemic zebrafish by atorvastatin is notably consistent with the known pharmaceutical effects of this drug on human beings. These results improve our understanding of the antiatherosclerotic effect of drugs. We envisage that our approach has the potential to become a platform to predict the pharmaceutical effects of new drugs aiming to cure human atherosclerotic diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|