Release of the Non-Steroidal Anti-Inflammatory Drug Flufenamic Acid by Multiparticulate Delivery Systems Promotes Adipogenic Differentiation of Adipose-Derived Stem Cells
Autor: | Anca Hermenean, Andreea D. Lazar, Sorina Dinescu, Marieta Costache, Mădălina G. Albu-Kaya, Sami Gharbia |
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Rok vydání: | 2020 |
Předmět: |
Adipose tissue
Cell morphology lcsh:Technology Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine flufenamic acid Adipocyte medicine multiparticulate drug delivery systems General Materials Science lcsh:Microscopy lcsh:QC120-168.85 030304 developmental biology 0303 health sciences lcsh:QH201-278.5 lcsh:T Chemistry adipose tissue engineering Cell biology perilipin Flufenamic acid lcsh:TA1-2040 Adipogenesis 030220 oncology & carcinogenesis Drug delivery Perilipin PPARγ2 lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering adipose-derived stem cells lcsh:Engineering (General). Civil engineering (General) adipogenic differentiation Wound healing lcsh:TK1-9971 medicine.drug |
Zdroj: | Materials Volume 13 Issue 7 Materials, Vol 13, Iss 1550, p 1550 (2020) |
ISSN: | 1996-1944 |
DOI: | 10.3390/ma13071550 |
Popis: | Engineered tissue-like structures often instigate an inflammatory response in the host that can inhibit wound healing and ultimately lead to the rejection of the implant. In our previous study, we have characterized the properties and biocompatibility of novel multiparticulate drug delivery systems (MDDS), based on collagen matrix with gradual release of anti-inflammatory drug flufenamic acid, we evaluated their anti-inflammatory potential and demonstrated their efficiency against burns and soft tissue lesions. In addition to these results, FA was previously described as a stimulant for adipogenesis, therefore we hypothesized that MDDS might also be appropriate for adipose tissue engineering. After the cell-scaffold constructs were obtained, cell morphology, adhesion and spreading on the systems were highlighted by scanning electron microscopy, immunostaining and confocal microscopy. The effect of FA-enriched materials on adipogenesis was evaluated at gene and protein level, by RT-qPCR, confocal microscopy and immunohistochemistry. Our current work indicates that flufenamic acid plays a beneficial role in adipocyte differentiation, with a direct effect upon the gene and protein expression of important early and late markers of adipogenesis, such as PPAR&gamma 2 and perilipin. |
Databáze: | OpenAIRE |
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