A Simple and Rapid in Vitro Bioassay for Inhibin*
| Autor: | Henry G. Burger, Helen Quigg, Russell S. Scott |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Biology Gonadotropin-Releasing Hormone Endocrinology Anterior pituitary Pituitary Gland Anterior Internal medicine Testis medicine Animals Humans Bioassay Inhibins Cells Cultured Sheep Dose-Response Relationship Drug Inhibin secretion Proteins In vitro Rats Testicular Hormones medicine.anatomical_structure Biological Assay Lymph Folliculogenesis Follicle Stimulating Hormone Spermatogenesis hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Endocrinology. 107:1536-1542 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-107-5-1536 |
| Popis: | This report describes a quantitative bioassay for inhibin which can be used to monitor purification and establish the physiological role of this hormone in spermatogenesis and folliculogenesis. Anterior pituitary cells from adult male Sprague-Dawley rats were dispersed and precultured for 18 h before the addition of inhibin-containing materials to the culture medium. After a further 72 h in culture, the medium was removed by aaspiration, and the cells were lysed releasing their intracellular hormone into RIA buffer. Cell content of FSH was reduced in inhibin-containing preparations, but without changes in LH, GH, and PRL concentrations. The inhibin dose-response curve, based on the inhibition of FSH in 15 experiments using an ovine testicular lymph preparation as a standard, had indices of precision between -0.032 and -0.098, and Finney's g ranged from 0.003--0.025. The interassay variability ranged from 15.0--16.9%. The assay had a practical capacity of 300--400 wells, which permitted the measurement of dose-response curves of 15 or more unknowns with quadruplicate wells per dose. The potency of unknown preparations was calculated with reference to the inhibin standard, which had a designated potency of 1 U/mg. Preparations showing nonparallelism were excluded. This assay presented advantages over those described previously, since it showed specificity of response to FSH and accommodated a large number of samples without loss of precision or reproducibility. It is therefore suitable for measurement of the inhibin-like activity in some biological fluids. |
| Databáze: | OpenAIRE |
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