Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goiás, Brazil
| Autor: | T. S. Silva, Marcia Dias, Ariel Eurides Stella, K. A. Nascimento, E.G. Freitas-Filho, Jefferson Fernando Naves Pinto, Marcos Roberto Alves Ferreira, C. N. Moreira |
|---|---|
| Přispěvatelé: | Universidade Federal de Goiás (UFG), Univ Fed Pelotas, Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Wet season Veterinary medicine phylogenetic STEC groups animal diseases Microorganism 030106 microbiology Biology medicine.disease_cause 03 medical and health sciences fluids and secretions Shiga toxin-producing Escherichia coli (STEC) phylogenetic STEC groups STEC reservoirs STX2 Dry season STEC reservoirs medicine Stx1 Stx2 Escherichia coli Gene Feces grupos STEC filogenéticos lcsh:SF1-1100 General Veterinary Phylogenetic tree Shiga toxin-producing Escherichia coli (STEC) Escherichia coli produtora de toxina shiga (STEC) 030104 developmental biology bacteria lcsh:Animal culture Stx1 reservatórios STEC Stx2 |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 70, Iss 6, Pp 1807-1813 Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.70 n.6 2018 Arquivo brasileiro de medicina veterinária e zootecnia Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| ISSN: | 1678-4162 0102-0935 |
| DOI: | 10.1590/1678-4162-9861 |
| Popis: | Made available in DSpace on 2019-10-04T18:54:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-11-01. Added 1 bitstream(s) on 2021-07-15T14:35:18Z : No. of bitstreams: 1 S0102-09352018000601807.pdf: 700373 bytes, checksum: a4b85729791d8af9f81524e8516d6c72 (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study determined the distribution of stx1 and stx2 genes in Escherichia coli isolated from dairy herds with regard to animal age, season, and farm production-scale, and analyzed the phylogenetic distribution of the groups A, B1, B2, and D of 276 isolates of bovine feces Shiga toxin-producing E. coli (STEC). The stx1 profile was the most common, detected in 20.4% (202/990) of the isolates, followed by stx2 (4.54%, 45/990) and stx1+stx2 (2.92%, 29/990). The stx1 gene was detected more frequently in calves than in adult animals. In the dry season (winter), the presence of stx1+stx2 profile in cattle feces was higher than in the rainy season (summer), while no significant changes were observed between seasons for the stx1 and stx2 profiles. The most predominant phylogenetic groups in adult animals were B1, A, and D, while groups A and B1 prevailed in calves. Our data highlight the importance of identifying STEC reservoirs, since 7.5% of the tested isolates were positive for stx2, the main profile responsible for the hemolytic-uremic syndrome (HUS). Moreover, these microorganisms are adapted to survive even in hostile environments and can contaminate the food production chain, posing a significant risk to consumers of animal products. Univ Fed Goias, Jatai, GO, Brazil Univ Fed Pelotas, Pelotas, RS, Brazil Univ Sao Paulo, Sao Paulo, SP, Brazil Univ Estadual Paulista, Jaboticabal, SP, Brazil Univ Estadual Paulista, Jaboticabal, SP, Brazil CNPq: 503886/2009-2 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|