Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5
| Autor: | Marcia Soares Vidal, Stefan Schwab, Helena Montano, Gilda Rose S. Amaral, Rosane Silva, Leonardo França, Marcio Alves-Ferreira, Cristian A. Alvarez Rojas, Rodolpho Mattos Albano, Marcio Martins Loureiro, Vânia L. Muniz de Pádua, Jacyara Maria Brito Macedo, Marcelo Bertalan, Flávia Alvim Dutra de Freitas, José Antônio Fernandes de Macêdo, Anna Neves, Alan T. Branco, Orlando B. Martins, H. V. Guedes, Ana Beatriz Furlanetto Pacheco, Leticia M. S. Lery, Gilberto Sachetto-Martins, Ana Coelho, Marcela Dreschsel, Viviane de Souza Magalhães, Luc Felicianus Marie Rouws, Daniel Figueiredo, Daniela Palermo de Carvalho, Eduardo de Matos Nogueira, Alessandra Rael Pereira, Adriana Silva Hemerly, Paulo Mascarello Bisch, Jadier de Oliveira Cunha Junior, K. R. S. Teixeira, Daniela Anhel de Paula Cidade, Luiz Fernando Bessa Seibel, José Ivo Baldani, Turán P. Ürményi, Shaila C. Rössle, Melissa Lemos, Beatriz Rossetti Ferreira, Wanda Almeida von Kruger, Gonçalo Apolinário de Souza Filho, Wellington Almeida, Tatiana de Almeida Simão, Paulo Cavalcanti Gomes Ferreira, Paula Gonzalez, Denise Neves de Oliveira, Luciana Pozzer, Jean Luiz Simões de Araújo, Paulo Sergio Torres Brioso, Alexander M. Cardoso, Heloisa Carneiro da Rocha Guillobel, Eidy de O. Santos, André Luiz Martinez de Oliveira, Carlos Henrique Salvino Gadelha Meneses, Elisete Pains Rodrigues, Sylvia Maria Campbell Alquéres, Ana Valadão, Victor Martin Quintana Flores, Edson Rondinelli |
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| Jazyk: | angličtina |
| Předmět: |
DNA
Bacterial lcsh:QH426-470 Genomic Islands lcsh:Biotechnology Molecular Sequence Data Endophyte Genome lcsh:TP248.13-248.65 Nitrogen Fixation Botany Genetics Symbiosis Gene Whole genome sequencing Comparative Genomic Hybridization Genomic Library biology fungi food and beverages Sequence Analysis DNA biology.organism_classification Saccharum Quorum sensing lcsh:Genetics Gluconacetobacter Horizontal gene transfer Nitrogen fixation Genome Bacterial Research Article Biotechnology |
| Zdroj: | BMC Genomics BMC Genomics, Vol 10, Iss 1, p 450 (2009) |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/1471-2164-10-450 |
| Popis: | Background Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications. |
| Databáze: | OpenAIRE |
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