Histamine alters cadherin-mediated sites of endothelial adhesion
| Autor: | Yih-Tai Chen, Sandra S. Shasby, Anant Kamath, D. Michael Shasby, Dana R. Ries, Michael C. Winter |
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| Rok vydání: | 1999 |
| Předmět: |
Pulmonary and Respiratory Medicine
Umbilical Veins Physiology Gene Expression CHO Cells Biology Transfection Umbilical vein Capillary Permeability chemistry.chemical_compound Physiology (medical) Cricetinae medicine Cell Adhesion Electric Impedance Animals Edema Humans Cell adhesion Cell Line Transformed Fluorescent Dyes Cadherin Cell Biology Adhesion Cadherins Cell biology Endothelial stem cell Apposition medicine.anatomical_structure chemistry Immunology Calcium Endothelium Vascular Fura-2 Histamine Blood vessel |
| Zdroj: | The American journal of physiology. 277(5) |
| ISSN: | 0002-9513 |
| Popis: | We tested the hypothesis that histamine alters the focal apposition of endothelial cells by acting on sites of cadherin-mediated cell-cell adhesion. Focal apposition was measured as the impedance of a cell-covered electrode, which was partitioned into a cell-matrix resistance, a cell-cell resistance, and membrane capacitance. Histamine causes an immediate, short-lived decrease in the impedance of an electrode covered with human umbilical vein endothelial (HUVE) cells. ECV304 cells are a line of spontaneously transformed HUVE cells that do not express the endothelial cadherin, cadherin-5. Histamine increased ECV304 cell calcium to 600 nM. Histamine did not increase myosin light chain phosphorylation of control or transfected ECV304 cells. ECV304 cells transfected with either E-cadherin or cadherin-5 on a dexamethasone-responsive plasmid (pLKneo) increased their cell-cell resistance when stimulated with dexamethasone, whereas ECV304 cells transfected with pLKneo-lacZ did not. Histamine did not affect the impedance of ECV304 cells transfected with pLKneo-lacZ. In contrast, histamine decreased the cell-cell resistance of ECV304 cells transfected with either pLKneo-E-cadherin or pLKneo-cadherin-5. From these data, we conclude that histamine acts on sites of cadherin-mediated cell-cell apposition. |
| Databáze: | OpenAIRE |
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