Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics
| Autor: | Débora Ferreira, Maria G. Sande, Lígia R. Rodrigues, Carla J. S. M. Silva, Joana Lúcia Lima Correia Rodrigues |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2021 |
| Předmět: |
2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19) diagnosis Computer science Point-of-Care Systems biorecognition Aptamer Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enzymes Clinical Biochemistry aptamers Biosensing Techniques Review 02 engineering and technology Computational biology biosensor Diagnostic tools Aptamers Antibodies 03 medical and health sciences Diagnosis antibodies Biorecognition 030304 developmental biology 0303 health sciences Science & Technology peptide nucleic acids Bacteria Reproducibility of Results pathogens General Medicine 021001 nanoscience & nanotechnology Enzymes DNAzymes 3. Good health Viruses peptides Pathogens Peptides Peptide nucleic acids 0210 nano-technology TP248.13-248.65 Biosensor Biotechnology Alternative strategy |
| Zdroj: | Biosensors Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Biosensors, Vol 11, Iss 418, p 418 (2021) |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios11110418 |
| Popis: | Infectious agents, especially bacteria and viruses, account for a vast number of hospitalisations and mortality worldwide. Providing effective and timely diagnostics for the multiplicity of infectious diseases is challenging. Conventional diagnostic solutions, although technologically advanced, are highly complex and often inaccessible in resource-limited settings. An alternative strategy involves convenient rapid diagnostics which can be easily administered at the point-of-care (POC) and at low cost without sacrificing reliability. Biosensors and other rapid POC diagnostic tools which require biorecognition elements to precisely identify the causative pathogen are being developed. The effectiveness of these devices is highly dependent on their biorecognition capabilities. Naturally occurring biorecognition elements include antibodies, bacteriophages and enzymes. Recently, modified molecules such as DNAzymes, peptide nucleic acids and molecules which suffer a selective screening like aptamers and peptides are gaining interest for their biorecognition capabilities and other advantages over purely natural ones, such as robustness and lower production costs. Antimicrobials with a broad-spectrum activity against pathogens, such as antibiotics, are also used in dual diagnostic and therapeutic strategies. Other successful pathogen identification strategies use chemical ligands, molecularly imprinted polymers and Clustered Regularly Interspaced Short Palindromic Repeats-associated nuclease. Herein, the latest developments regarding biorecognition elements and strategies to use them in the design of new biosensors for pathogens detection are reviewed. This research is affiliated with the VibrANT project that received funding from the EU Horizon 2020 Research and Innovation Programme under the Marie Sklowdowska-Curie Grant, agreement no 765042. In addition, the authors acknowledge the financial support from Fundação para a Ciência e Tecnologia (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit. Débora Ferreira (DF) is the recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15) funded by the European Social Fund under the scope of Norte2020. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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