Isolation and Genome Sequencing of a Novel Pseudoalteromonas Phage PH1
| Autor: | Yong Jiang, Yan Li, Yaoyuan Zhang, Xue Meng, Zhaoyang Liu, Hongbing Shao, Min Wang, Duobing Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Phage display 030106 microbiology Genome Viral Biology Applied Microbiology and Biotechnology Microbiology Genome Host Specificity DNA sequencing Bacteriophage Open Reading Frames 03 medical and health sciences Marine bacteriophage Pseudoalteromonas Bacteriophages Seawater ORFS Genetics Base Composition Temperature Virion DNA Sequence Analysis DNA General Medicine Hydrogen-Ion Concentration Podoviridae biology.organism_classification 030104 developmental biology Lytic cycle DNA Viral |
| Zdroj: | Current Microbiology. 74:212-218 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-016-1175-9 |
| Popis: | The family Pseudoalteromonas is highly adaptable to dissimilar ecological habitats and plays an important ecological role in the marine environment. In this study, a new Pseudoalteromonas phage PH1 was isolated from the Yellow Sea. To better understand the bacteriophage, its biological properties, including morphology, host range, growth phenotype, thermal and pH stability, and nucleic acid composition, were investigated in detail. The result showed that the phage PH1 is a Podoviridae-phage with an icosahedral head (60 nm of diameter) and a short tail (26 nm in length). The phage PH1 genome consists of 42,685 bp length double-stranded DNA with a G+C content of 42.24% and is predicted to have 55 open reading frames (ORFs) with an average length of 740 bp nucleotides each. The phage PH1 genome adds a new Podoviridae-phage genome to marine bacteriophage dataset, which will provide useful basic information for further molecular research on interaction mechanisms between bacteriophages and their hosts. |
| Databáze: | OpenAIRE |
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