Isolation and identification of chitin from heavy mineralized skeleton of Suberea clavata (Verongida: Demospongiae: Porifera) marine demosponge
| Autor: | Marcin Wysokowski, Mikhail V. Tsurkan, Heike Meissner, Roberta Galli, Teofil Jesionowski, Vasilii V. Bazhenov, Nicole J. de Voogd, Murat Kaya, Esra Bulut, Hermann Ehrlich, Cécile Debitus |
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| Přispěvatelé: | Jayakumar, R (ed.), Meenakshi, S. (ed.), Mühendislik Fakültesi, Ehrlich, Hermann -- 0000-0003-3654-0810, Bazhenov, Vasilii -- 0000-0002-1346-1151, Debitus, Cecile -- 0000-0002-3978-0644, Tsurkan, Mikhail V. -- 0000-0003-4000-3890, Kaya, Murat -- 0000-0001-6954-2703 |
| Rok vydání: | 2016 |
| Předmět: |
Chitin
02 engineering and technology Test (biology) Calcofluor-white Biology 01 natural sciences Biochemistry chemistry.chemical_compound Demosponge Structural Biology Botany Animals Suberea clavata sponges Molecular Biology Skeleton Suberea Clavata Minerals 010405 organic chemistry Chitinases General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Isolation (microbiology) 0104 chemical sciences Porifera chemistry Sponges Chitinase biology.protein Identification (biology) 0210 nano-technology |
| Zdroj: | International journal of biological macromolecules. 104 |
| ISSN: | 1879-0003 |
| Popis: | WOS: 000412959500037 PubMed: 28185932 Since the discovery of chitin in skeletal structures of sponges (Porifera) in 2007, studies on search of novel species which possess this structural aminopolysaccharide continue up today. The most potential source of chitin is suggested to be localized in the four families of sponges related to the order Verongida (Demospongiae) which nevertheless require further clarification. Here, we report for the first time the isolation and identification of alpha-chitin from the Suberea clavata demosponge (Aplysinidae: Verongida). Raman spectroscopy, Calcofluor White staining, chitinase test and ESI-MS techniques were used to identify chitin. We suggest that the presence of chitin within fibrous skeletons of diverse species of Verongida order, and, especially in all species of the Aplysinidae family, may be useful for the identification of novel, previously unidentified marine demosponges. (C) 2017 Elsevier B.V. All rights reserved. DFG (Germany) [EH 394-3]; PUT research grant (Poland) [03/32/DSPB/0706/2017] This work was supported by DFG Grant EH 394-3 (Germany) and PUT research grant 03/32/DSPB/0706/2017 (Poland). The authors thank Prof. Dr. Yixin Zhang for the use of the ESI-MS instrument and Rob van Soest for his support and comments. |
| Databáze: | OpenAIRE |
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