The effects of superoxide dismutase knockout on the oxidative stress parameters and survival of mouse erythrocytes
| Autor: | Ewa Macierzyńska, Łukasz Piotrowski, Agnieszka Grzelak, Marcin Kruszewski, Grzegorz Bartosz, Łukasz Pułaski, Błażej Rychlik |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Erythrocytes Cell Survival Short Communication SOD1 medicine.disease_cause Biochemistry Superoxide dismutase Mice Internal medicine Blood plasma Heinz bodies medicine Animals Molecular Biology chemistry.chemical_classification Mice Knockout Reactive oxygen species biology Chemistry Superoxide Dismutase Cell Biology Organ Size Blood proteins Erythrocyte Red blood cell Oxidative Stress Endocrinology medicine.anatomical_structure biology.protein Erythrocyte Count Acetylcholinesterase Oxidation-Reduction Oxidative stress Spleen Heinz body |
| Zdroj: | Cellular & Molecular Biology Letters |
| ISSN: | 1689-1392 |
| Popis: | The erythrocytes of 12-month old Sod1 −/− mice showed an increased level of reactive oxygen species (ROS), as estimated by the degree of dihydroethidine and dihydrorhodamine oxidation, and the increased level of Heinz bodies. No indices of severe oxidative stress were found in the red blood cells and blood plasma of Sod1 −/− mice as judged from the lack of significant changes in the levels of erythrocyte and plasma glutathione, plasma protein thiol and carbonyl groups and thiobarbituric-acid reactive substances in the blood plasma. However, a decreased erythrocyte lifespan, increased reticulocyte count and splenomegaly were noted, indicating the importance of superoxide dismutase for maintaining erythrocyte viability. The levels of erythrocyte ROS and Heinz bodies and the reticulocyte count were indistinguishable in Sod1 +/+ and Sod1 +/− mice, suggesting that a superoxide dismutase activity decrease to half of its normal value may be sufficient to secure the protective effects of the enzyme. |
| Databáze: | OpenAIRE |
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