Identification of glucose regulated protein94 (GRP94) in filarial parasite S. cervi and its expression under ER stress
| Autor: | Anchal Singh, Faiyaz Ahmad, Shweta Sharma, Sushma Rathaur |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
biology
Physiology Setaria Nematode Endoplasmic reticulum Membrane Proteins Helminth Proteins Tunicamycin Endoplasmic Reticulum Stress biology.organism_classification Biochemistry Hsp90 Molecular biology Brugia malayi chemistry.chemical_compound chemistry parasitic diseases Setaria cervi Unfolded protein response biology.protein Animals HSP70 Heat-Shock Proteins Loa loa Endoplasmin Molecular Biology |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 258:110683 |
| ISSN: | 1096-4959 |
| Popis: | GRP94, a member of HSP90 family, is involved in folding and degradation of endoplasmic reticulum proteins. The proteome analysis of Setaria cervi, a bovine filarial parasite showed that a 91 kDa protein was over expressed, after the parasites were maintained in glucose deprived medium. The MALDI- LC/MS analysis of the 91 kDa band confirmed it as endoplasmin precursor (GRP94). Amino acid sequence alignment of S.cervi GRP94 exhibited maximum similarity with human filarial parasite Wuchereria bancrofti, Brugia malayi and Loa loa GRP94. Tunicamycin treatment of S. cervi worms revealed that the expression of GRP94 is associated with ER stress. Transcription of S. cervi grp94 as well as igf is regulated by transcription factors ATF-6 and XBP-1S which was confirmed by Real Time PCR. Moreover, marked alteration in the expression of igf after 3 h and 6 h of drug treatment suggested propagation of survival pathway under ER stress. The activities of ER stress markers protein disulphide isomerase and glycosyltransferase were significantly reduced after 6 h of tunicamycin treatment. The present findings thus indicate that the expression of GRP94 and regulation of its expression is under ER stress in Setaria cervi. To our knowledge this is the first report of identification of GRP94, in any filarial parasite till date. |
| Databáze: | OpenAIRE |
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