Colorimetric oligonucleotide array for genotyping of hepatitis C virus based on the 5' non-coding region
| Autor: | Honglian Zhang, Shi-min Gu, Kangdong Liu, Jianlong Zhao, Hongju Mao, Gendi Jin, Zhimeng Lu, Mengsu Yang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Genes
Viral Genotype Hepatitis C virus Clinical Biochemistry Hepacivirus Biology medicine.disease_cause Biochemistry Sensitivity and Specificity DNA sequencing Interferon medicine Genotyping Oligonucleotide Array Sequence Analysis Genetics Base Sequence Oligonucleotide Biochemistry (medical) General Medicine medicine.disease Virology Hepatocellular carcinoma Colorimetry DNA microarray 5' Untranslated Regions medicine.drug |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 388(1-2) |
| ISSN: | 0009-8981 |
| Popis: | Background Hepatitis C virus infection is now one of the most important causes of chronic liver disease. HCV genotype has emerged as an important factor both in predicting a sustained response to, and in determining the duration of, antiviral therapy. Microarrays provide excellent tools for rapid genotyping of hepatitis C virus due to their ability to perform a multitude of tests simultaneously. Methods A colorimetric oligonucleotide array that specially and rapidly detects the genotype/subtypes of hepatitis C virus was designed and developed. Results A total of 256 consecutive HCV RNA positive samples derived from patients with chronic hepatitis C were examined for their genotypes/subtypes by the oligonucleotide array. Identification of genotypes and subtypes by the HCV oligonucleotide array showed a concordance rate of 99.2% with nucleotide sequencing results. Conclusion The oligonucleotide array offers an economical and convenient alternative to detect HCV infection, as well as permitting identification of the genotype/subtypes, in a large-scale setting. Oligonucleotide arrays provide a reliable and sensitive method for the detection and discrimination of HCV genotypes common in China, and are suitable for routine use in a diagnostic laboratory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect