Quantitative and qualitative normative dataset for intraepidermal nerve fibers using skin biopsy

Autor: Collongues, N. (Nicolas), Lacombe, B. (Brigitte), Mutter, C. (Catherine), Chamard-Witkowski, L. (Ludivine), Debouverie, M. (Marc), Chanson, J. (Jean-baptiste), Antal, M. (Maria), Benardais, K. (Karelle), De Seze, J. (Jerome), Velten, M. (Michel), Boehm, N. (Nelly), Sommer, C. (Claudia) (editor)
Přispěvatelé: Biopathologie de la Myéline, Neuroprotection et Stratégies Thérapeutiques (BMNST), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
Databases
Factual
Biopsy
lcsh:Medicine
Biochemistry
Epithelium
Merkel Cells
Nerve Fibers
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
030212 general & internal medicine
lcsh:Science
Skin
Neurons
Multidisciplinary
medicine.diagnostic_test
Peripheral Nervous System Diseases
Dermis
Anatomy
Middle Aged
Immunohistochemistry
Healthy Volunteers
medicine.anatomical_structure
Creatinine
Cytochemistry
Female
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
France
Cellular Types
Integumentary System
Merkel cell
Immunocytochemistry
Research Article
Adult
Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]
Small Fiber Neuropathy
Surgical and Invasive Medical Procedures
Nerve fiber
03 medical and health sciences
medicine
Humans
Leg
business.industry
Papillary dermis
Bright-field microscopy
lcsh:R
Biology and Life Sciences
Epithelial Cells
Cell Biology
Axons
Apposition
Biological Tissue
Cellular Neuroscience
Skin biopsy
lcsh:Q
Epidermis
business
Biomarkers
Databases
Chemical
030217 neurology & neurosurgery
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2018, 13 (1), pp.e0191614. ⟨10.1371/journal.pone.0191614⟩
PLoS ONE, 2018, 13 (1), pp.e0191614. ⟨10.1371/journal.pone.0191614⟩
PLoS ONE, Vol 13, Iss 1, p e0191614 (2018)
ISSN: 1932-6203
Popis: Background Skin biopsy is the most relevant tool to diagnose small-fiber neuropathy. A well-documented normal dataset for intraepidermal nerve fiber in the distal leg is required to improve its diagnostic value. Methods Three hundred healthy subjects were enrolled in the study, after clinical and biological screening to exclude neurological and systemic pathologies. A distal leg biopsy was taken and intraepidermal nerve fiber density after protein gene product-9.5 immunocytochemistry with brightfield microscopy was determined. Morphological variations of intraepidermal nerve fibers, previously described in small-fiber neuropathies, were analyzed. One hundred biopsies were also analyzed at the ultrastructural level. Findings The median number of fibers was lower in men compared to women and decreased with age. Using statistical modeling taking into account age and gender, we calculated the 5th percentile of intraepidermal nerve fiber density as follows: 7.6156–0.0769 x age (years) + 1.5506 x gender (woman = 1; man = 0). We observed a low frequency of large swellings or horizontal branchings but an increasing frequency of small swellings of intraepidermal nerve fibers and irregular distribution along the dermal-epidermal junction with age. Axonal diameter of unmyelinated fibers of the papillary dermis did not vary with age or gender. Ultrastructural analysis also showed that fiber endings in close apposition to Merkel cells should not be mistaken for small-fiber swellings. Conclusions Our dataset allows accurate calculation of the normal density of intraepidermal nerve fibers for each year of age and provides original morphological observations that improve the diagnostic value of skin biopsy in the distal leg for small-fiber neuropathy.
Databáze: OpenAIRE
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