Effects of Cooling Time on Membrane Integrity and Motility of Frozen-Thawed Canine Spermatozoa using Two Different Commercial Egg Yolk-based Extenders at Two Different Cooldown Equilibration Times
| Autor: | J.A. Len, Bruce E. Eilts, S du Bois, J. M. Parlevliet |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
endocrine system Time Factors food.ingredient Motility Semen Semen analysis Biology urologic and male genital diseases law.invention Andrology Dogs fluids and secretions Endocrinology food law Yolk Freezing medicine Animals Sperm motility Cryopreservation medicine.diagnostic_test urogenital system Cell Membrane Extender Egg Yolk Spermatozoa Sperm Cooling time Cold Temperature Semen Analysis Sperm Motility Animal Science and Zoology Semen Preservation Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 47:278-280 |
| ISSN: | 0936-6768 |
| DOI: | 10.1111/rda.12018 |
| Popis: | Two commercially available egg yolk-based semen extenders, one marketed for human semen freezing (HEYE) and one marketed for canine semen freezing (CEYE), were used to cryopreserve semen from single ejaculates of 11 different dogs. For each extender, a 30- and a 60-min cooldown period was used prior to the addition of the extender containing glycerol and then immediately frozen in liquid nitrogen vapours. Sperm motility was measured using a computer-assisted semen analysis (CASA) system. Sperm intact membranes were measured using SYBER-14 and propidium iodide. Semen in the HEYE cooled for 60 min had a significantly greater percentage of intact membranes than the semen in the HEYE cooled for 30 min (p = 0.02). Semen in the HEYE cooled for 60 min had significantly greater total motility (p = 0.007) and progressive motility (p = 0.004) than semen cooled for 60 min in the CEYE and semen cooled for 30 min in the HEYE (total motility p = 0.02 and progressive motility p = 0.02). Semen cooled for 60 min in the CEYE did not differ significantly in total (p = 0.6) or progressive motility (p = 0.4) than semen cooled for 30 min in the CEYE. There was no difference in total (p = 0.8) or progressive motility (p = 0.8) between the semen cooled for 30 min in the HEYE and the semen cooled for 30 min in the CEYE. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text