Effect of different metals on oxidative state and mitochondrial membrane potential in trout erythrocytes
| Autor: | Sonia Silvestri, Luca Tiano, Giancarlo Falcioni, Francesca Brugè, Patrick Orlando |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Membrane potential Reactive oxygen species 030102 biochemistry & molecular biology Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health chemistry.chemical_element Context (language use) General Medicine Zinc Oxidative phosphorylation medicine.disease_cause Pollution Redox 03 medical and health sciences 030104 developmental biology chemistry Biochemistry Toxicity medicine Oxidative stress |
| Zdroj: | Ecotoxicology and environmental safety. |
| ISSN: | 1090-2414 |
| Popis: | Homeostasis of metal ions is critical for life and excessive exposure can promote cellular damage that could be due to oxidative damage. In this context we evaluated the effects of three different elements (copper, zinc and aluminum) on oxidative stress and mitochondrial functionality in nucleated trout erythrocytes (Oncorhynchus mykiss). Flowcytometric measurements using MitoProbe and DCFDA-H2 as fluorescent probes, indicated that redox active copper was able to influence all the biological parameters considered while redox inert, zinc and aluminum, show no significant effects. Toxicity of Al and Zn represent a debated argument and their ability to interact with other endogenous metal ions/metal binding proteins could play a role modulating their cellular toxicity. |
| Databáze: | OpenAIRE |
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