Ganoderma lucidum Extract Reduces the Motility of Breast Cancer Cells Mediated by the RAC–Lamellipodin Axis
| Autor: | Ariana C Acevedo-Diaz, Gabriela Ortiz-Soto, Ivette J. Suarez-Arroyo, Michelle M. Martínez Montemayor, Astrid Zayas-Santiago |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell signaling Motility lcsh:TX341-641 CDC42 Ganoderma lucidum Immunofluorescence Metastasis 03 medical and health sciences 0302 clinical medicine breast cancer medicine Viability assay Nutrition and Dietetics medicine.diagnostic_test Chemistry Brief Report cancer cell migration lamellipodin medicine.disease 3. Good health Rac 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Cancer research Lamellipodium lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients Nutrients, Vol 11, Iss 5, p 1116 (2019) |
| ISSN: | 2072-6643 |
| Popis: | Breast cancer (BC) is the second leading cause of cancer death among women worldwide. The main cause of BC morbidity and mortality is the invasiveness capacity of cancer cells that may lead to metastasis. Here, we aimed to investigate the therapeutic efficacy of Ganoderma lucidum extract (GLE)—a medicinal mushroom with anticancer properties—on BC motility via the Rac/Lamellipodin pathway. GLE treatment effects were tested on MDA-MB-231 breast cancer cells. The effects were tested on cell viability, migration and invasion. Pulldowns, immunoblotting, and immunofluorescence were used to measure Rac activity and the expression of proteins involved in cell migration and in lamellipodia formation, respectively. As a result, GLE suppressed BC cell viability, migration, and invasion capacity. GLE impaired Rac activity, as well as downregulated Lamellipodin, ENA/VASP, p-FAK (Tyr925), Cdc42, and c-Myc expression. Lamellipodia formation was significantly reduced by GLE. In conclusion, we demonstrate that GLE reduces Rac activity and downregulates signaling molecules involved in lamellipodia formation. These novel findings serve as basis for further studies to elucidate the potential of GLE as a therapeutic agent regulating the Rac/Lamellipodin pathway in BC metastasis. |
| Databáze: | OpenAIRE |
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