Differences in embryonic gene expression and quality indicate the benefit of epidermal growth factor receptor inhibitor during prematuration to improve competence in bovine oocytes
| Autor: | Patricia Kubo Fontes, Priscila Chediek Dall’Acqua, Giovana Barros Nunes, Márcia Marinho, Marcelo Fábio Gouveia Nogueira, Gisele Zoccal Mingoti, Cíntia Rodrigues da Silva, Flavia L. Lopes |
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| Rok vydání: | 2018 |
| Předmět: |
Homeobox protein NANOG
Embryonic Development Fertilization in Vitro Andrology Endocrinology Gene expression medicine Animals Epidermal growth factor receptor Enzyme Inhibitors EGFR inhibitors biology Epidermal Growth Factor Embryogenesis Gene Expression Regulation Developmental Tyrphostins Oocyte Embryo Mammalian In Vitro Oocyte Maturation Techniques medicine.anatomical_structure Apoptosis biology.protein Quinazolines Animal Science and Zoology Cattle Female Embryo quality Biotechnology |
| Zdroj: | Reproduction in domestic animals = Zuchthygiene. 54(4) |
| ISSN: | 1439-0531 |
| Popis: | The epidermal growth factor receptor (EGFR) pathway is directly involved in oocyte meiotic resumption induced by a gonadotropic stimulus. Here, we used an EGFR inhibitor (AG1478) to inhibit spontaneous meiosis resumption in bovine oocytes (EGFR- group) during 8 hr prematuration and assessed the competence of such oocytes for embryonic development, apoptosis and gene expression in comparison with Control group which was not prematured. Data are presented as mean ± SEM. Blastocysts rate on day 7 (40.81%, averaged) and hatching rate on day 9 (77.35%, averaged) were unaffected by treatment (p > 0.05). Similarly, treatment did not affect (p > 0.05) the total cell number on day 7 (119.05, averaged) and on day 9 (189.5, averaged). Apoptosis was reduced (p |
| Databáze: | OpenAIRE |
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