Microbial translocation and T cell activation are modified by direct-acting antiviral therapy in HCV-infected patients
Autor: | Gianluca Mennini, Clementina Maria Galluzzo, Silvia Baroncelli, Lucia Palmisano, Adriano De Santis, Stefano Ginanni Corradini, M. Lupo, Massimo Rossi, Barbara Lattanzi, Zuleika Michelini, Manuela Merli |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Male
0301 basic medicine T-Lymphocytes medicine.medical_treatment intestinal fatty acid‐binding protein Hepacivirus Liver transplantation medicine.disease_cause Immunotherapy Adoptive Gastroenterology Pathogenesis Liver disease 0302 clinical medicine Fibrosis microbial translocation hepatitis C direct‐acting antiviral soluble‐CD14 lipopolysaccharide‐binding protein CD38+ HLA‐DR lipopolysaccharide binding protein Pharmacology (medical) Longitudinal Studies Alanine Transaminase Hepatitis C Middle Aged Female 030211 gastroenterology & hepatology Adult medicine.medical_specialty Hepatitis C virus Fatty Acid-Binding Proteins Antiviral Agents lipopolysaccharide‐binding protein Transaminase 03 medical and health sciences Internal medicine medicine Humans Aged Hepatitis Hepatology business.industry Hepatitis C Chronic medicine.disease soluble‐CD14 Liver Transplantation 030104 developmental biology business Biomarkers |
Popis: | Background Microbial translocation from the gut lumen has been involved in the pathogenesis of liver damage in hepatitis C virus (HCV) infection. Aim To investigate the impact of direct-acting antiviral treatment on microbial translocation and T-cell activation, in patients with hepatitis C-related liver disease. Methods We enrolled two groups of HCV-infected patients undergoing direct-acting antiviral treatment: patients with fibrosis ≥F3 according to Metavir (Group ≥F3); patients with hepatitis C recurrence after liver transplantation and Metavir ≥F2 (Group Liver Transplantation + ≥F2). All patients were treated with direct-acting antivirals based on ongoing guidelines. Surrogate biomarkers of microbial translocation (plasma concentrations of soluble-CD14, lipopolysaccharide-binding protein and intestinal fatty acid-binding protein) were evaluated at baseline, at first month, at the end of treatment and 3 months later. T-cell activation was measured by expression of CD38+ HLA-DR at the same time points, only in Group ≥F3. Results There were 32 patients in Group ≥F3 and 13 in Group LT + ≥F2. At baseline, levels of soluble-CD14 and lipopolysaccharide-binding protein were significantly higher in both groups vs healthy controls. Baseline soluble-CD14 correlated with glutamic-oxalacetic transaminase (r = 0.384, P = 0.009) and glutamic-pyruvic transaminase (r = 0.293, P = 0.05). A significant decrease in plasma levels of surrogate microbial translocation biomarkers was observed during and after treatment in the two groups although values were not normalised. In Group ≥F3, CD38+ HLADR+ T-cell expression was significantly decreased by direct-acting antiviral treatment. Relapsers (9%) showed higher soluble-CD14 levels at baseline. Conclusion Surrogate microbial translocation markers and T cell activation are increased in HCV-infected patients with liver fibrosis and decrease during direct-acting antiviral treatment. |
Databáze: | OpenAIRE |
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