The protective effects of omega-6 fatty acids in experimental autoimmune encephalomyelitis (EAE) in relation to transforming growth factor-beta 1 (TGF-β1) up-regulation and increased prostaglandin E2 (PGE2) production

Autor: Sandra Amor, M M Morris-Downes, Lorna Layward, Laurence S. Harbige, Dudley C. Dumonde
Rok vydání: 2000
Předmět:
Male
Encephalomyelitis
Autoimmune
Experimental

T-Lymphocytes
Linoleic acid
Neuroimmunology
Molecular Sequence Data
Immunology
Gene Expression
Dinoprostone
Myelin oligodendrocyte glycoprotein
Transforming Growth Factor beta1
Interferon-gamma
Mice
chemistry.chemical_compound
Dietary Fats
Unsaturated

Transforming Growth Factor beta
Fatty Acids
Omega-6

Concanavalin A
medicine
Animals
Plant Oils
Immunology and Allergy
Amino Acid Sequence
gamma-Linolenic Acid
Cells
Cultured

chemistry.chemical_classification
biology
alpha-Linolenic acid
Cell Membrane
Fatty Acids
Experimental autoimmune encephalomyelitis
alpha-Linolenic Acid
Fatty acid
medicine.disease
Up-Regulation
Myelin-Associated Glycoprotein
chemistry
Eicosanoid
Fatty Acids
Unsaturated

biology.protein
Interleukin-2
Myelin-Oligodendrocyte Glycoprotein
Arachidonic acid
Interleukin-4
Mitogens
Myelin Proteins
Spleen
Polyunsaturated fatty acid
Zdroj: Clinical and Experimental Immunology. 122:445-452
ISSN: 1365-2249
0009-9104
DOI: 10.1046/j.1365-2249.2000.01399.x
Popis: SUMMARYPolyunsaturated fatty acids are known to affect the immune response and administration of the omega-6 fatty acid linoleic acid has been reported to be beneficial in multiple sclerosis (MS) and EAE. In this study we have investigated the effects of oral feeding of plant lipid rich in the omega-6 fatty acid gamma-linolenic acid from Borago officinalis on acute and relapse disease and the immune response in EAE using SJL mice. EAE was induced by an encephalitogenic peptide (92–106) of myelin oligodendrocyte glycoprotein (MOG), and mice were fed the plant lipid daily from 7 days after EAE induction to assess the effects on acute disease and from day 25 to assess the effects on disease relapse. The clinical incidence and histological manifestations of acute EAE, and the clinical relapse phase of chronic relapsing EAE (CREAE) were markedly inhibited by omega-6 fatty acid feeding. A significant increase in the production of TGF-β1 in response to concanavalin A (Con A) at day 13 and a significant increase in TGF-β1 and PGE2 to Con A, PPD and MOG peptide (92–106) at day 21 were detected in spleen mononuclear cells from fatty acid-fed mice. There was no difference in interferon-gamma, IL-4 and IL-2 production between the fatty acid-fed and control groups. Significantly higher TGF-β mRNA expression was found in the spleens of omega-6 fatty acid-fed mice at day 21. There were no differences in spleen cell proliferative response to Con A, PPD and MOG peptide (92–106). Biochemical analysis of spleen cell membrane fatty acids revealed significant increases in the eicosanoid precursor fatty acids dihomo-γ-linolenic acid and arachidonic acid in response to gamma-linolenic acid feeding, indicating rapid metabolism to longer chain omega-6 fatty acids. These results show that oral feeding of gamma-linolenic acid-rich plant lipid markedly affects the disease course of acute EAE and CREAE and is associated with an increase in cell membrane long chain omega-6 fatty acids, production of PGE2 and gene transcription and, on activation, secretion of TGF-β1.
Databáze: OpenAIRE
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