Diversity and Multiplicity of P. falciparum infections among asymptomatic school children in Mbita, Western Kenya
| Autor: | Victor A. Mobegi, Abdoulie O. Touray, Jeremy K. Herren, Fred Wamunyokoli |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Adolescent Molecular biology Plasmodium falciparum 030231 tropical medicine lcsh:Medicine Diseases Biology Asymptomatic Article Loss of heterozygosity 03 medical and health sciences 0302 clinical medicine Multiplicity of infection parasitic diseases Genetics medicine Humans Parasite hosting Malaria Falciparum lcsh:Science Child Asymptomatic Infections Molecular Epidemiology Genetic diversity Multidisciplinary lcsh:R Genetic Variation DNA Protozoan medicine.disease Kenya Virology 030104 developmental biology Child Preschool Population study lcsh:Q Female medicine.symptom Asymptomatic carrier Malaria Microsatellite Repeats |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-62819-w |
| Popis: | Multiplicity of infection (MOI) and genetic diversity of P. falciparum infections are important surrogate indicators for assessing malaria transmission intensity in different regions of endemicity. Determination of MOI and diversity of P. falciparum among asymptomatic carriers will enhance our understanding of parasite biology and transmission to mosquito vectors. This study examined the MOI and genetic diversity of P. falciparum parasite populations circulating in Mbita, a region characterized as one of the malaria hotspots in Kenya. The genetic diversity and multiplicity of P. falciparum infections in 95 asymptomatic school children (age 5–15 yrs.) residing in Mbita, western Kenya were assessed using 10 polymorphic microsatellite markers. An average of 79.69% (Range: 54.84–95.74%) of the isolates analysed in this study were polyclonal infections as detected in at least one locus. A high mean MOI of 3.39 (Range: 2.24–4.72) and expected heterozygosity (He) of 0.81 (Range: 0.57–0.95) was reported in the study population. The analysed samples were extensively polyclonal infections leading to circulation of highly genetically diverse parasite populations in the study area. These findings correlated with the expectations of high malaria transmission intensity despite scaling up malaria interventions in the area thereby indicating the need for a robust malaria interventions particularly against asymptomatic carriers in order to attain elimination in the region. |
| Databáze: | OpenAIRE |
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