The Anatomy of the Mitochondrial DNA. The Localization of the Heat-Induced and RNAase-Induced Scissions in the Phosphodiester Backbones
| Autor: | Jürgen Koch, Harald H. Porcher |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Mitochondrial DNA
Hot Temperature Phosphorous Acids Cesium DNA Single-Stranded Biology Tritium DNA Mitochondrial Biochemistry Cell Line Mice chemistry.chemical_compound Ribonucleases D-loop Heavy strand Chlorides Centrifugation Density Gradient Animals Pancreas Hydrolysis Esters Haplorhini Molecular biology chemistry Cell culture Phosphodiester bond Vero cell Nucleic Acid Conformation DNA Circular Thymidine Ultracentrifugation |
| Zdroj: | European Journal of Biochemistry. 40:329-336 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1973.tb03201.x |
| Popis: | The distribution of the mitochondrial DNA among the allomorphic forms, supercoiled circle, open circle and linear rod is a characteristic feature of each cell line. After pulse-chase labeling with [Me-3H]thymidine the bulk of the radioactivity can be located either in the supercoiled circular mitochondrial DNA (monkey Vero, ATCC; CCL 81) or in the open circular mitochondrial DNA (mouse NCTC 929, ATCC; CCL 1). The open circular mitochondrial DNA from Vero cells contains one single nick, which is located in the heavy strand (alkaline CsCl gradient). In contrast, the open circular mitochondrial DNA from mouse cells contains one nick in one strand and at least two nicks in the opposite strand. The bulk of the supercoiled Vero mitochondrial DNA has a heat-labile site in the heavy strand and one site in both strands at which the phosphodiester backbone can be hydrolyzed by pancreatic RNAase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|