Activation of Virus-specific Memory B Cells in the Absence of T Cell Help
| Autor: | Peter Rohwer, Karin Klenovsek, Andrea Schneider, Michael Mach, Thomas Winkler, Uwe Ritter, Barbara J. Hebeis |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
adoptive transfer
T cell Immunology B-cell receptor Naive B cell Cytomegalovirus Lymphocyte Activation Lymphocyte Depletion Article immunological memory Mice medicine Animals Humans Immunology and Allergy Cytotoxic T cell B lymphocyte memory Antigen-presenting cell B cell Homeodomain Proteins Mice Knockout B-Lymphocytes CD40 antigen-specific immunity biology T-Lymphocytes Helper-Inducer Molecular biology B-1 cell Disease Models Animal medicine.anatomical_structure Antibody Formation Cytomegalovirus Infections biology.protein Immunologic Memory Gene Deletion |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.20030091 |
| Popis: | Humoral immunity is maintained by long-lived plasma cells, constitutively secreting antibodies, and nonsecreting resting memory B cells that are rapidly reactivated upon antigen encounter. The activation requirements for resting memory B cells, particularly the role of T helper cells, are unclear. To analyze the activation of memory B cells, mice were immunized with human cytomegalovirus, a complex human herpesvirus, and tick-born encephalitis virus, and a simple flavivirus. B cell populations devoid of Ig-secreting plasma cells were adoptively transferred into T and B cell–deficient RAG-1−/− mice. Antigenic stimulation 4–6 d after transfer of B cells resulted in rapid IgG production. The response was long lasting and strictly antigen specific, excluding polyclonal B cell activation. CD4+ T cells were not involved since (a) further depletion of CD4+ T cells in the recipient mice did not alter the antibody response and (b) recipient mice contained no detectable CD4+ T cells 90 d posttransfer. Memory B cells could not be activated by a soluble viral protein without T cell help. Transfer of memory B cells into immunocompetent animals indicated that presence of helper T cells did not enhance the memory B cell response. Therefore, our results indicate that activation of virus-specific memory B cells to secrete IgG is independent of cognate or bystander T cell help. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|