The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo
| Autor: | Ursula Weber, Angel-Carlos Roman, Brian Ciruna, Aurore Dussert, Marek Mlodzik, Giovanna M. Collu, Sophie Balmer, Jose Maria Carvajal-Gonzalez, Meg Mendoza |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Frizzled
Adaptor Protein Complex 1 Dishevelled Proteins General Physics and Astronomy GTPase Biology DNA-binding protein Clathrin General Biochemistry Genetics and Molecular Biology Article Animals Genetically Modified Cell polarity Animals Drosophila Proteins Wings Animal Early Growth Response Protein 2 Zebrafish Adaptor Proteins Signal Transducing MyoD Protein Multidisciplinary Cadherin Cell Polarity Gene Expression Regulation Developmental Membrane Proteins General Chemistry LIM Domain Proteins Zebrafish Proteins Cadherins Phosphoproteins Frizzled Receptors Cell biology DNA-Binding Proteins AP-1 Complex biology.protein Clathrin adaptor proteins ADP-Ribosylation Factor 1 Drosophila Carrier Proteins |
| Popis: | A key step in generating planar cell polarity (PCP) is the formation of restricted junctional domains containing Frizzled/Dishevelled/Diego (Fz/Dsh/Dgo) or Van Gogh/Prickle (Vang/Pk) complexes within the same cell, stabilized via Flamingo (Fmi) across cell membranes. Although models have been proposed for how these complexes acquire and maintain their polarized localization, the machinery involved in moving core PCP proteins around cells remains unknown. We describe the AP-1 adaptor complex and Arf1 as major regulators of PCP protein trafficking in vivo. AP-1 and Arf1 disruption affects the accumulation of Fz/Fmi and Vang/Fmi complexes in the proximo-distal axis, producing severe PCP phenotypes. Using novel tools, we demonstrate a direct and specific Arf1 involvement in Fz trafficking in vivo. Moreover, we uncover a conserved Arf1 PCP function in vertebrates. Our data support a model whereby the trafficking machinery plays an important part during PCP establishment, promoting formation of polarized PCP-core complexes in vivo. |
| Databáze: | OpenAIRE |
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