Streamlined preparation of genomic DNA in agarose plugs for pulsed-field gel electrophoresis
| Autor: | Eugene Kroll, Emily Cook, Karen H. Schmidt, Jacob Childress, Charlotte M. van der Graaf, Linda D. Hicks, Frank Rosenzweig |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Gel electrophoresis
deep-well 48-well plate Chromatography Computer science chromosomal DNA preparation karyotyping bacterial infections and mycoses chemistry.chemical_compound genomic DNA chemistry pulsed-field gel electrophoresis genomic fingerprinting Pulsed-field gel electrophoresis Protocol General Earth and Planetary Sciences Agarose Chromosomal dna Typing DNA plugs General Environmental Science |
| Zdroj: | Journal of Biological Methods |
| ISSN: | 2326-9901 |
| Popis: | Genome analysis using pulsed-field gel electrophoresis (PFGE) has been used in applications ranging from typing bacterial strains to radiobiology to cancer research. While methods for running PFGE have been significantly improved since its invention, the method for preparing chromosomal DNA itself has remained essentially unchanged. This limits the applicability of PFGE, especially when analyses require many samples. We have streamlined sample preparation for routine applications of PFGE through the use of deep-well 48-well plates. Besides saving time, our protocol has the added advantage of reducing the volume of expensive reagents. Our improved protocol enables us to reduce throughput time and simplify the procedure, facilitating wider application of PFGE-based analyses in the laboratory. |
| Databáze: | OpenAIRE |
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