Biodegradation Potential and Diversity of Diclofenac-degrading Microbiota in an Immobilized Cell Biofilter
| Autor: | George Tsiamis, Nikolaos Remmas, Dimitrios G. Karpouzas, Spyridon Ntougias, Paraschos Melidis, Efstathia Navrozidou |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Bioengineering 010501 environmental sciences lcsh:Chemical technology 01 natural sciences lcsh:Chemistry priority substances Rhodanobacter 03 medical and health sciences Wickerhamiella Chemical Engineering (miscellaneous) lcsh:TP1-1185 Food science 0105 earth and related environmental sciences pH acidification biology Chemistry Process Chemistry and Technology Biodegradation biology.organism_classification wastewater treatment stomatognathic diseases 030104 developmental biology Activated sludge lcsh:QD1-999 Wastewater Granulicella Biofilter Parvibaculum lavamentivorans Sewage treatment Bordetella petrii Rhodopseudomonas palustris |
| Zdroj: | Processes Volume 7 Issue 9 Processes, Vol 7, Iss 9, p 554 (2019) |
| ISSN: | 2227-9717 |
| DOI: | 10.3390/pr7090554 |
| Popis: | Despite that diclofenac has been embodied to the European watch list of priority substances of concern, studies on diclofenac biodegradation are limited and the diversity of diclofenac-degrading microbiota remains unknown. In this work, an immobilized cell biofilter was constructed and operated to evaluate its effectiveness to depurate high strength diclofenac wastewater and to identify the diclofenac-degrading community accommodated in activated sludge by employing high-throughput sequencing techniques. After a two-month adaptation period, biofilter removal efficiencies reached values as high as 97.63 ± 0.62%, whereas utilization of diclofenac in the immobilized cell biofilter led to a drastic pH decrease. Based on Illumina sequencing, the major bacterial taxa identified in the immobilized cell biofilter were members of the species Granulicella pectinivorans and Rhodanobacter terrae, followed by members of the species Castellaniella denitrificans, Parvibaculum lavamentivorans, Bordetella petrii, Bryocella elongata and Rhodopseudomonas palustris. The ability of such taxa to utilize a wide range of carbon sources and to effectively adapt under acidic conditions seemed to be the main parameters, which favored their prevalence in the immobilized cell biofilter. In addition, Wickerhamiella was the predominant fungal taxon in the immobilized cell biofilter, which appears to be actively involved in diclofenac degradation in activated sludge systems. |
| Databáze: | OpenAIRE |
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