Microbial transformation of immunosuppressive compounds III. Glucosylation of immunomycin (FR 900520) and FK 506 byBacillus subtilis ATCC 55060
| Autor: | Richard L. Monaghan, Tom S. Chen, Francis J. Dumont, Annjia Hsu, B. Petuch, Byron H. Arison |
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| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Time Factors Glycosylation Chromatography Bacillaceae biology Metabolite Bioengineering Biological activity Bacillus subtilis biology.organism_classification Applied Microbiology and Biotechnology Bacillales Tacrolimus carbohydrates (lipids) chemistry.chemical_compound Enzyme chemistry Biochemistry Biotransformation Glucosyltransferases Immunosuppressive Agents Biotechnology |
| Zdroj: | Journal of Industrial Microbiology. 13:131-135 |
| ISSN: | 1476-5535 0169-4146 |
| DOI: | 10.1007/bf01584111 |
| Popis: | The regiospecific glucosylation of FK 506 and immunomycin (FR 900520) at the 24-hydroxy position was performed using resting cells of Bacillus subtilis ATCC 55060. 24-Glucopyranosyl FK 506 and 24-glucopyranosyl immunomycin were isolated by methylene chloride extraction and purification using reverse phase HPLC. The metabolite structures were established using spectroscopic techniques including MS and NMR. The glucose conjugate was further confirmed by chemical degradation. Enzymatic glucosylation was demonstrated using cell-free extracts derived from Bacillus subtilis ATCC 55060. The 24-glucosyltransferase, which appears UDP-glucose dependent, was solubilized from cell membranes by treatment with 0.1% Nonidet P-40 detergent. The optimal conditions for assay of the enzyme have been determined. |
| Databáze: | OpenAIRE |
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