The spectral properties of DNA and RNA macromolecules at low temperatures: fundamental and applied aspects
| Autor: | Valeriy M. Yashchuk, Vladislav Yu. Kudrya |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
02 engineering and technology
010402 general chemistry 01 natural sciences Fluorescence Quantitative Biology::Subcellular Processes chemistry.chemical_compound General Materials Science Singlet state Instrumentation Spectroscopy Quantitative Biology::Biomolecules Chemistry Temperature RNA DNA 021001 nanoscience & nanotechnology Quantitative Biology::Genomics Atomic and Molecular Physics and Optics 0104 chemical sciences Telomere Luminescent Measurements Biophysics Absorption (chemistry) 0210 nano-technology Thymidine Macromolecule |
| Zdroj: | Methods and applications in fluorescence. 5(1) |
| ISSN: | 2050-6120 |
| Popis: | This paper summarizes the results of studies of the spectral properties-optical absorption, fluorescence and phosphorescence-of DNA and RNA macromolecules and synthetic poly-, oligo- and mono-nucleotides, which have been carried out in our laboratory. The system of first excited singlet and triplet energy levels for DNA and RNA is evaluated using low-temperature (4.2 K-77 K) luminescent measurements. The traps of the singlet and triplet electronic excitations in these compounds are identified. An important self-protection mechanism against photo-damage of DNA and RNA by UV photons or penetrative radiation based on the capture of triplet electronic-energy excitations by the most photostable centers-in DNA, the complex formed by neighboring adenosine (A) and thymidine (T) links; in RNA, the adenosine links-is described. It is confirmed that despite similarities in the chemical and partly energy structures DNA is more stable than RNA. The spectral manifestation of the telomeres (the important functional system) in DNA macromolecules is examined. The results obtained on telomere fragments provide the possibility of finding the configuration peculiarities of the triplet excitations traps in DNA macromolecules. The resulting spreading length of the migrating singlet (l s) and triplet (l t) excitations for DNA and RNA macromolecules are evaluated. |
| Databáze: | OpenAIRE |
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