Antimicrobial activity and toxicity of glass ionomer cement containing an essential oil
Autor: | Isabela Albuquerque Passos Farias, Jocianelle Maria Félix Fernandes Nunes, Thays Matias Ribeiro, Fábio Correia Sampaio, V.A. Menezes, C.A. Vieira |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Medicine (General) Physiology QH301-705.5 Immunology Biophysics Ocean Engineering Biochemistry Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration 0302 clinical medicine R5-920 Materials Testing Oils Volatile Humans Agar diffusion test Food science General Pharmacology Toxicology and Pharmaceutics Biology (General) Thymol Minimum bactericidal concentration biology Chemistry General Neuroscience Biofilm Cell Biology General Medicine Antimicrobial biology.organism_classification 030104 developmental biology 030220 oncology & carcinogenesis Biofilms Glass ionomer cements Anti-infective agents Artemia salina Research Article |
Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 53, Iss 12 (2020) Brazilian Journal of Medical and Biological Research v.53 n.12 2020 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 53, Issue: 12, Article number: e9468, Published: 30 OCT 2020 |
Popis: | The aim of this study was to evaluate the antimicrobial activity and toxicity of glass ionomer cement (GIC) modified with 5-methyl-2-(1-methylethyl)phenol (thymol) against Streptococcus mutans in silico and in vitro. The antimicrobial activity of thymol on GIC modified with concentrations of 2% (GIC-2) and 4% (GIC-4) was evaluated in a model of planktonic cell biofilm using agar diffusion test, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), dynamic biofilm (continuous flow cell parallel), and bacterial kinetics. Conventional GIC (GIC-0) was used as a control. Thymol toxicity was evaluated in Artemia salina and in silico using Osiris® software. Differences between groups were estimated by analysis of variance, followed by Tukey post hoc test, with a 5% significance level. The results of the agar diffusion test between groups were not significantly different (P≥0.05). Thymol had potential bacteriostatic and bactericidal activity against Streptococcus mutans with respect to planktonic growth, with MIC of 100 µg/mL and MBC of 400 µg/mL. The groups GIC-0, GIC-2, and GIC-4 reduced the biofilm by approximately 10, 85, and 95%, respectively. Bacterial kinetics showed efficiency of the modified GICs for up to 96 h. GIC with thymol was effective against S. mutans, with significant inhibition of the biofilms. Analyses in silico and using Artemia salina resulted in no relevant toxicity, suggesting potential for use in humans. GIC-2 was effective against S. mutans biofilm, with decreased cell viability. |
Databáze: | OpenAIRE |
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