Improved preparation of group-specific component (Gc) protein to derive macrophage activating factor
| Autor: | Xuyang Li, Rong Wang, Tomonari Muramatsu, Sachiko Takahashi, Masaru Tanokura, Yuki Morita, Satoshi Hachimura, Takuya Miyakawa, Masumi Ueda |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Vitamin D-binding protein Angiogenesis Chemistry medicine.medical_treatment Vitamin D-Binding Protein Macrophage-activating factor 01 natural sciences GcMAF Chromatography Affinity 03 medical and health sciences Immune system Affinity chromatography Cancer immunotherapy Biochemistry Macrophage-Activating Factors 010608 biotechnology Protein purification medicine Humans 030304 developmental biology Biotechnology |
| Zdroj: | Protein expression and purification. 175 |
| ISSN: | 1096-0279 |
| Popis: | Cancer immunotherapy has recently attracted attention as an approach for cancer treatment through the activation of the immune system. Group-specific component (Gc) protein is a precursor for macrophage activating factor (GcMAF), which has a promising immunomodulatory effect on the suppression of tumor growth and angiogenesis. In this study, we successfully purified Gc protein from human serum using anion-exchange chromatography combined with affinity chromatography using a 25-OH-D3-immobilized column. The purity of Gc protein reached 95.0% after anion-exchange chromatography. The known allelic variants of Gc protein are classified into three subtypes-Gc1F, Gc1S and Gc2. The fragment sequence of residues 412-424 determined according to their MS/MS spectra is available to evaluate the subtypes of Gc protein. The data showed that the Gc protein purified in this study consisted of the Gc1F and Gc2 subtypes. Our method improved the purity of Gc protein, which was not affected by the treatment to convert it into GcMAF using β-galactosidase- or neuraminidase-immobilized resin, and will be useful for biological studies and/or advanced clinical uses of GcMAF, such as cancer immunotherapy. |
| Databáze: | OpenAIRE |
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